Almalki Esam, Al-Shaebi Esam M, Al-Quarishy Saleh, El-Matbouli Mansour, Abdel-Baki Abdel-Azeem S
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Department of Medical Laboratory, College of Applied Medical Sciences, Majmaah University, Saudi Arabia.
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Saudi J Biol Sci. 2017 Jan;24(1):90-94. doi: 10.1016/j.sjbs.2016.05.007. Epub 2016 May 14.
Hydatid disease is an important economic and human public health problem with a wide geographical distribution. Surgical excision remains the primary treatment and the only hope for complete cure of hydatosis. The most important complications arising from surgical excision, however, is recurrence, which is due to dissemination of protoscolices during the surgery. Pre-surgical inactivation of the contents of the hydatid cyst by injection of scolicidal agent into the cyst has been used as adjunct to surgery in order to overcome the risk of recurrence. In the present study, ethanolic extracts of turmeric () and ginger () were tested as scolicidal agent for protoscoleces. Protoscoleces were collected aseptically from sheep livers containing hydatid cysts. Three concentrations (10, 30 and 50 mg/ml) of each extract were investigated and viability of the protoscoleces was tested by 0.1% eosin staining. Ginger extract showed the strongest scolicidal effect (100%) after 20 min at a concentration of 30 mg/ml and 10 min at 50 mg/ml The maximum scolicidal effect of turmeric was 93.2% after 30 min at a concentration of 50 mg/ml. It is concluded that turmeric and ginger extracts have high scolicidal activity and could be used as effective scolicidal agents against protoscoleces.
包虫病是一个重要的经济和人类公共卫生问题，地理分布广泛。手术切除仍然是主要治疗方法，也是治愈包虫病的唯一希望。然而，手术切除引发的最重要并发症是复发，这是由于手术过程中原头节的播散所致。通过向包虫囊肿内注射杀头节剂对囊肿内容物进行术前灭活已被用作手术辅助手段，以降低复发风险。在本研究中，测试了姜黄（）和生姜（）的乙醇提取物作为针对原头节的杀头节剂。从含有包虫囊肿的绵羊肝脏中无菌收集原头节。研究了每种提取物的三种浓度（10、30和50毫克/毫升），并通过0.1%伊红染色测试原头节的活力。生姜提取物在浓度为30毫克/毫升时20分钟后显示出最强的杀头节效果（100%），在浓度为50毫克/毫升时10分钟后显示出最强效果。姜黄在浓度为50毫克/毫升时30分钟后的最大杀头节效果为93.2%。得出的结论是，姜黄和生姜提取物具有较高的杀头节活性，可作为针对原头节的有效杀头节剂。
