Thangamani Saravanan, Huang Jing, Hart Charles E, Guzman Hilda, Tesh Robert B
Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, Texas.
Am J Trop Med Hyg. 2016 Nov 2;95(5):1169-1173. doi: 10.4269/ajtmh.16-0448. Epub 2016 Aug 29.
Previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. In this study, designed to test whether Zika virus (ZIKV) could be vertically transmitted, female Aedes aegypti and Aedes albopictus were injected with ZIKV, and their F adult progeny were tested for ZIKV infection. Six of 69 Ae. aegypti pools, comprised of a total of 1,738 F adults, yielded ZIKV upon culture, giving a minimum filial infection rate of 1:290. In contrast, none of 803 F Ae. albopictus adults (32 pools) yielded ZIKV. The MFIR for Ae. aegypti was comparable to MFIRs reported for other flaviviruses in mosquitoes, including dengue, Japanese encephalitis, yellow fever, West Nile, and St. Louis encephalitis viruses. The results suggest that vertical transmission may provide a potential mechanism for the virus to survive during adverse conditions.
先前的实验研究表明，多种蚊媒传播的黄病毒病原体可在其昆虫媒介中垂直传播，为这些虫媒病毒在不利气候条件下或在缺乏易感脊椎动物宿主的情况下持续存在提供了一种机制。在本旨在测试寨卡病毒（ZIKV）是否可垂直传播的研究中，对雌性埃及伊蚊和白纹伊蚊注射ZIKV，并对其F代成年子代进行ZIKV感染检测。69个埃及伊蚊样本池中的6个，总共包含1738只F代成虫，培养后产生了ZIKV，最低子代感染率为1:290。相比之下，803只白纹伊蚊F代成虫（32个样本池）均未产生ZIKV。埃及伊蚊的最低子代感染率与报道的蚊子中其他黄病毒（包括登革热、日本脑炎、黄热病、西尼罗河病毒和圣路易斯脑炎病毒）的最低子代感染率相当。结果表明，垂直传播可能为病毒在不利条件下存活提供一种潜在机制。
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