Uhlen Mathias, Bandrowski Anita, Carr Steven, Edwards Aled, Ellenberg Jan, Lundberg Emma, Rimm David L, Rodriguez Henry, Hiltke Tara, Snyder Michael, Yamamoto Tadashi
Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden.
Center for Research in Biological Systems, University of California at San Diego, San Diego, California, USA.
Nat Methods. 2016 Oct;13(10):823-7. doi: 10.1038/nmeth.3995. Epub 2016 Sep 5.
We convened an International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual ‘pillars’ for antibody validation to be used in an application-specific manner.
我们召集了一个国际抗体验证工作组,以制定用于常见研究应用中抗体验证的最佳方法,并提供确保抗体可重复性的指南。我们建议以特定应用的方式使用五个概念性的抗体验证“支柱”。