Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Illinois, USA.
Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
Sci Rep. 2016 Sep 6;6:32922. doi: 10.1038/srep32922.
Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid growth and proliferation, cancer cells not only alter the fatty acid synthesis and metabolism intracellularly and extracellularly, but also in the macroenvironment via direct or indirect pathways. We report here, using Raman micro-spectroscopy, that an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats. By minimizing confounding effects from mixed chemicals and optimizing the signal-to-noise ratio of Raman spectra, we observed a large-scale transition from monounsaturated fatty acids to PUFAs in the tumor while only a small subset of fatty acids transitioned to PUFAs in the tumor micro- and macroenvironment. These data have important implications for further clarifying the macroenvironmental effect of cancer progression and provide new potential approaches for characterizing the tumor micro- and macroenvironment of breast cancer in both pre-clinical animal studies and clinical applications.
脂肪酸在癌细胞的生长和转移中发挥着重要作用。为了促进其旺盛的生长和增殖，癌细胞不仅在细胞内和细胞外改变脂肪酸的合成和代谢，而且还通过直接或间接途径改变宏观环境中的脂肪酸。我们在这里报告，使用拉曼微光谱技术，在从乳腺癌患者和荷瘤大鼠获得的癌组织和正常外观的乳腺组织中都发现了多不饱和脂肪酸（PUFAs）产量的增加。通过最小化混合化学物质的干扰影响并优化拉曼光谱的信噪比，我们观察到在肿瘤中从单不饱和脂肪酸到 PUFAs 的大规模转变，而在肿瘤微环境和宏观环境中只有一小部分脂肪酸转变为 PUFAs。这些数据对进一步阐明癌症进展的宏观环境效应具有重要意义，并为在临床前动物研究和临床应用中描述乳腺癌的肿瘤微环境和宏观环境提供了新的潜在方法。
