Zhu Fu-Yuan, Chan Wai-Lung, Chen Mo-Xian, Kong Ricky P W, Cai Congxi, Wang Qiaomei, Zhang Jian-Hua, Lo Clive
School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong, China.
School of Life Sciences, The Chinese University of Hong Kong , Shatin, Hong Kong, China.
J Proteome Res. 2016 Oct 7;15(10):3528-3539. doi: 10.1021/acs.jproteome.6b00258. Epub 2016 Sep 6.
Lead (Pb) pollution is a growing environment problem that continuously threatens the productivity of crops. To understand the molecular mechanisms of plant adaptation to Pb toxicity, we examined proteome changes in Arabidopsis seedlings following Pb treatment by SWATH-MS, a label-free quantitative proteomic platform. We identified and quantified the expression of 1719 proteins in water- and Pb-treated plants. Among them, 231 proteins showed significant abundance changes (151 elevated and 80 reduced) upon Pb exposure. Functional categorization revealed that most of the Pb-responsive proteins are involved in different metabolic processes. For example, down-regulation of photosynthesis and biosynthesis of isoprenoids and tetrapyrroles in chloroplasts were observed. On the contrary, pathways leading to glutathione, jasmonic acid (JA), glucosinolate (GSL), and phenylpropanoid production are up-regulated. Experimental characterizations demonstrated a rapid elevation of endogenic JA production in Pb-treated Arabidopsis seedlings, while a JA-deficient mutant and a JA-insensitive mutant showed hypersensitivity to root inhibition by Pb, implicating an essential role of JA during Pb responses. Consistently, methyl jasmonate supplementation alleviated Pb toxicity in the wild-type and JA-deficient mutant. Furthermore, GSL levels were substantially enhanced following Pb treatment, while such induction was not detected in the JA mutant, suggesting that the Pb-induced GSL accumulation is JA-dependent. Overall, our work represents the first SWATH-MS analysis in Arabidopsis and highlights a potential mediating role of JA during Pb stress.
铅(Pb)污染是一个日益严重的环境问题,持续威胁着作物的生产力。为了解植物适应铅毒性的分子机制,我们通过SWATH-MS(一种无标记定量蛋白质组学平台)检测了拟南芥幼苗在铅处理后的蛋白质组变化。我们鉴定并定量了水处理和铅处理植物中1719种蛋白质的表达。其中,231种蛋白质在铅暴露后显示出显著的丰度变化(151种升高,80种降低)。功能分类显示,大多数铅响应蛋白参与不同的代谢过程。例如,观察到叶绿体中光合作用以及类异戊二烯和四吡咯生物合成的下调。相反,导致谷胱甘肽、茉莉酸(JA)、芥子油苷(GSL)和苯丙烷类化合物产生的途径上调。实验表征表明,铅处理的拟南芥幼苗中内源性JA产量迅速升高,而JA缺陷突变体和JA不敏感突变体对铅诱导的根抑制表现出超敏感性,这表明JA在铅响应过程中起重要作用。同样,茉莉酸甲酯的添加减轻了野生型和JA缺陷突变体中的铅毒性。此外,铅处理后GSL水平显著提高,而在JA突变体中未检测到这种诱导,这表明铅诱导的GSL积累是JA依赖性的。总体而言,我们的工作代表了在拟南芥中的首次SWATH-MS分析,并突出了JA在铅胁迫期间的潜在介导作用。