Duranti Sabrina, Gaiani Federica, Mancabelli Leonardo, Milani Christian, Grandi Andrea, Bolchi Angelo, Santoni Andrea, Lugli Gabriele Andrea, Ferrario Chiara, Mangifesta Marta, Viappiani Alice, Bertoni Simona, Vivo Valentina, Serafini Fausta, Barbaro Maria Raffaella, Fugazza Alessandro, Barbara Giovanni, Gioiosa Laura, Palanza Paola, Cantoni Anna Maria, de'Angelis Gian Luigi, Barocelli Elisabetta, de'Angelis Nicola, van Sinderen Douwe, Ventura Marco, Turroni Francesca
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Gastroenterology Unit, University Hospital of Parma, Via Gramsci 14, 43126 Parma, Italy.
FEMS Microbiol Ecol. 2016 Dec;92(12). doi: 10.1093/femsec/fiw191. Epub 2016 Sep 7.
Ulcerative colitis (UC) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. In this study, microbiota cataloging of UC patients by 16S rRNA microbial profiling revealed a marked reduction of bifidobacteria, in particular the Bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of UC. We investigated this further through an in vivo trial by testing the effects of oral treatment with B. bifidum PRL2010 in a wild-type murine colitis model. TNBS-treated mice receiving 10(9) cells of B. bifidum PRL2010 showed a marked reduction of all colitis-associated histological indices as well as maintenance of mucosal integrity as it was shown by the increase in the expression of many tight junction-encoding genes. The protective role of B. bifidum PRL2010, as well as its sortase-dependent pili, appears to be established through the induction of an innate immune response of the host. These results highlight the importance of B. bifidum as a microbial biomarker for UC, revealing its role in protection against experimentally induced colitis.
溃疡性结肠炎（UC）与特定肠道共生菌的显著改变有关，其中一些可能参与微生物群介导的保护作用。在本研究中，通过16S rRNA微生物谱分析对UC患者的微生物群进行分类，发现双歧杆菌明显减少，尤其是两歧双歧杆菌，这表明该分类群在UC的病因学中发挥生物学作用。我们通过体内试验进一步研究了这一现象，在野生型小鼠结肠炎模型中测试了口服两歧双歧杆菌PRL2010的效果。接受10⁹个两歧双歧杆菌PRL2010细胞的经三硝基苯磺酸（TNBS）处理的小鼠，所有与结肠炎相关的组织学指标均显著降低，并且许多紧密连接编码基因表达增加表明黏膜完整性得以维持。两歧双歧杆菌PRL2010及其分选酶依赖性菌毛的保护作用似乎是通过诱导宿主的先天免疫反应而确立的。这些结果突出了两歧双歧杆菌作为UC微生物生物标志物的重要性，揭示了其在抵御实验性诱导结肠炎中的作用。
