Oldereid N B, Rui H, Clausen O P, Purvis K
Andrology Laboratory, National Hospital, Oslo, Norway.
J Reprod Fertil. 1989 Jul;86(2):731-6. doi: 10.1530/jrf.0.0860731.
The sperm qualities of 350 men under fertility investigation were compared in relation to their smoking habits. The sperm variables included number, motility, morphology and vitality. Sperm motility was assessed objectively by laser-Doppler spectroscopy. In a randomly selected group, sperm samples were subjected to flow cytometry to assess the levels of DNA condensation. No significant differences (Kruskal-Wallis' test) in any aspect of sperm quality including DNA distribution could be demonstrated between non-smokers, moderate smokers (1-14 cigarettes/day) and heavy smokers (15-40 cigarettes/day). This was true when the data were pooled and when oligozoospermic/hypozoospermic ejaculates (1-39 x 10(6)/ml) and asthenozoospermic ejaculates (less than 25% of sperm cells with progressive movement) were analysed separately. The distribution of non-smokers, moderate and heavy smokers was the same in groups of men with normal sperm quality as those with impaired quality. The present study does not provide support for the contention that smoking has deleterious effects on sperm quality, at least using conventional parameters.
对350名接受生育能力调查的男性的精子质量与其吸烟习惯进行了比较。精子变量包括数量、活力、形态和存活率。精子活力通过激光多普勒光谱法进行客观评估。在一个随机选择的组中，对精子样本进行流式细胞术以评估DNA浓缩水平。在非吸烟者、中度吸烟者（每天1 - 14支香烟）和重度吸烟者（每天15 - 40支香烟）之间，在精子质量的任何方面（包括DNA分布）均未显示出显著差异（Kruskal-Wallis检验）。当数据汇总时以及分别分析少精子症/弱精子症射精（1 - 39×10⁶/毫升）和弱精子症射精（进行性运动的精子细胞少于25%）时，情况都是如此。精子质量正常的男性组与质量受损的男性组中，非吸烟者、中度吸烟者和重度吸烟者的分布相同。本研究不支持吸烟对精子质量有有害影响这一观点，至少使用传统参数时是这样。
