Engh E, Schølberg A, Clausen O P, Purvis K
Andrology Laboratory, National Hospital, Oslo, Norway.
Int J Fertil Menopausal Stud. 1993 Sep-Oct;38(5):305-10.
To determine the extent to which sperm chromatin varies in its degree of condensation in 53 apparently normozoospermic men and in 14 men of proven fertility.
DNA flow cytometry after staining of spermatozoa with ethidium bromide and mithramycin.
Normozoospermic men could be divided into four subgroups according to the fluorescence pattern of their sperm DNA. More than half of the normozoospermic group and all of those of proven fertility shared a similar profile with a narrow symmetrical peak of haploid fluorescence without significant sub-populations. Deviations from this pattern were generally not associated with any major differences in sperm quality.
(a) Current criteria for normozoospermia should be expanded to include additional tests of biochemical integrity. (b) Fertilizing ability appears to be associated with a relatively homogeneous profile of sperm DNA condensation.
确定53名明显精子正常的男性以及14名已证实具有生育能力的男性中精子染色质凝聚程度的差异程度。
用溴化乙锭和光神霉素对精子进行染色后进行DNA流式细胞术检测。
根据精子DNA的荧光模式，精子正常的男性可分为四个亚组。超过一半的精子正常组以及所有已证实具有生育能力的男性具有相似的特征，即单倍体荧光呈现狭窄的对称峰，没有明显的亚群。与这种模式的偏差通常与精子质量的任何主要差异无关。
（a）目前的正常精子症标准应扩大，以包括生化完整性的额外检测。（b）受精能力似乎与精子DNA凝聚的相对均匀特征有关。
