Robertson D A, Heagerty A M, Ollerenshaw J D, Swales J D
Department of Medicine, Leicester Royal Infirmary, UK.
J Hum Hypertens. 1989 Apr;3(2):117-23.
Ten normotensive subjects had their omnivore diet supplemented with increasing doses of linoleic acid in the form of safflower seed oil in order to examine the effects of this polyunsaturated fat upon leucocyte sodium transport. Increasing the dose ingested to the limits of tolerance produced a significant decrease in ouabain resistant sodium efflux (P less than 0.05) but no significant change in total or ouabain-sensitive sodium extrusion. Intraleucocytic sodium content was correlated with erythrocyte membrane oleic acid % (r = 0.368, P less than 0.05); leucocyte ouabain resistant flux was correlated with oleic acid % (r = 0.453, P less than 0.01) and linoleic acid % (r = -0.319, P less than 0.05). No such associations were observed with ouabain sensitive sodium extrusion. No changes in body weight or urinary electrolyte excretion were observed. It is concluded that, at physiological concentrations, membrane linoleic acid content influences transmembrane sodium fluxes but not through modulation of sodium pump activity. Furthermore, the beneficial effect of this dietary manoeuvre, observed previously with small increments of safflower seed oil, was not seen in this experiment so the hypotensive activity of this manipulation must be regarded as limited.
十名血压正常的受试者以红花籽油的形式摄入递增剂量的亚油酸，以补充其杂食性饮食，目的是研究这种多不饱和脂肪对白细胞钠转运的影响。将摄入剂量增加到耐受极限会导致哇巴因抗性钠外流显著降低（P小于0.05），但总钠或哇巴因敏感性钠排出量无显著变化。白细胞内钠含量与红细胞膜油酸百分比相关（r = 0.368，P小于0.05）；白细胞哇巴因抗性通量与油酸百分比相关（r = 0.453，P小于0.01）和亚油酸百分比相关（r = -0.319，P小于0.05）。未观察到与哇巴因敏感性钠排出量有此类关联。未观察到体重或尿电解质排泄的变化。得出的结论是，在生理浓度下，膜亚油酸含量影响跨膜钠通量，但不是通过调节钠泵活性来实现。此外，先前观察到的少量增加红花籽油的这种饮食操作的有益效果，在本实验中未见到，因此必须认为这种操作的降压活性是有限的。
