Nikitushkin V D, Shleeva M O, Zinin A I, Trutneva K A, Ostrovsky D N, Kaprelyants A S
A.N. Bach Institute of Biochemistry, Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky pr. 33, build. 2, 119071, Moscow, Russia
A.N. Bach Institute of Biochemistry, Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Sciences, Leninsky pr. 33, build. 2, 119071, Moscow, Russia.
FEMS Microbiol Lett. 2016 Oct;363(19). doi: 10.1093/femsle/fnw206. Epub 2016 Sep 8.
Upon transition of Mycobacterium smegmatis into the dormant state, accumulation of a dark brown fluorescent pigment was observed. This pigment gave bright red fluorescence in both cells and the culture medium. Based on H-NMR, MALDI and UV spectra, the fluorescent compounds, extracted from the culture medium as well as from the dormant cells, were concluded to be a mixture of free coproporphyrin III and uroporphyrin III and their corresponding methyl esters. A possible significance of porphyrin pigment accumulation in the dormant cells is discussed.
耻垢分枝杆菌进入休眠状态后，观察到一种深棕色荧光色素的积累。这种色素在细胞和培养基中均发出亮红色荧光。基于氢核磁共振、基质辅助激光解吸电离和紫外光谱，从培养基以及休眠细胞中提取的荧光化合物被确定为游离粪卟啉III和尿卟啉III及其相应甲酯的混合物。文中讨论了卟啉色素在休眠细胞中积累的可能意义。
