School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea.
Sungkyun Biotech Co. LTD., Suwon 16419, Korea.
Molecules. 2018 Oct 23;23(11):2732. doi: 10.3390/molecules23112732.
Wanger (Cornaceae) has been broadly used in traditional East Asian medicine for the treatment of various disorders, including skin inflammation and diarrhea. As part of our efforts to identify structurally and/or biologically new compounds from Korean medicinal plants, we have explored potentially new bioactive constituents from . In the present study, seven triterpenoids (⁻) were isolated from stems and stem bark. Compounds ⁻ were new tirucallane triterpenoids (cornusalterins N-P) and compounds ⁻ were isolated for the first time from . The structures of the new compounds were determined based on 1D and 2D NMR spectroscopic data interpretations and HR-ESIMS, as well as a computational method coupled with a statistical procedure (DP4+). The regulatory effects of the isolated triterpenoids (⁻) on mesenchymal stem cell (MSC) differentiation to adipocytes and osteoblasts were examined in the C3H10T1/2 cell line. Although these compounds had little effect on MSC differentiation to osteoblasts, lipid droplet formation in adipocyte-differentiated MSCs decreased in the presence of the seven triterpenoids. Compounds and each had a relatively distinct correlation between dose and efficacy, showing adipogenesis suppression at higher concentrations. Our findings demonstrate that the active compounds and can exert beneficial effects in regulation of adipocyte differentiation.
菝葜属（Cornaceae）在传统的东亚医学中被广泛用于治疗各种疾病，包括皮肤炎症和腹泻。作为我们从韩国药用植物中鉴定结构和/或生物新颖化合物的努力的一部分，我们从菝葜属植物中探索了具有潜在生物活性的新成分。在本研究中，从菝葜茎和茎皮中分离出七种三萜类化合物（⁻）。化合物 ⁻ 为新的羽扇豆烷三萜类化合物（cornusalterins N-P），化合物 ⁻ 为首次从菝葜属植物中分离得到。根据 1D 和 2D NMR 光谱数据解释和 HR-ESIMS 以及与统计程序（DP4+）耦合的计算方法，确定了新化合物的结构。在 C3H10T1/2 细胞系中，研究了分离得到的三萜类化合物（⁻）对间充质干细胞（MSC）向脂肪细胞和成骨细胞分化的调节作用。尽管这些化合物对 MSC 向成骨细胞的分化几乎没有影响，但在存在这七种三萜类化合物的情况下，脂肪细胞分化的 MSC 中脂质滴的形成减少了。化合物 和 之间具有相对明显的剂量与疗效相关性，在较高浓度下表现出脂肪生成抑制作用。我们的研究结果表明，活性化合物 和 可以在调节脂肪细胞分化中发挥有益作用。
