Godoy-Gallardo Maria, Manzanares-Céspedes Maria Cristina, Sevilla Pablo, Nart José, Manzanares Norberto, Manero José M, Gil Francisco Javier, Boyd Steven K, Rodríguez Daniel
Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark.
Unidad de Anatomía y Embriología Humana, Faculty of Dentistry, University of Barcelona, Barcelona, Spain.
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:538-45. doi: 10.1016/j.msec.2016.07.020. Epub 2016 Jul 9.
The aim of this study was to evaluate the in vivo effect of antibacterial modified dental implants in the first stages of peri-implantitis. Thirty dental implants were inserted in the mandibular premolar sites of 5 beagle dogs. Sites were randomly assigned to Ti (untreated implants, 10units), Ti_Ag (silver electrodeposition treatment, 10units), and Ti_TSP (silanization treatment, 10units). Coated implants were characterized by scanning electron microscopy, interferometry and X-ray photoelectron spectroscopy. Two months after implant insertion, experimental peri-implantitis was initiated by ligature placement. Ligatures were removed 2months later, and plaque formation was allowed for 2 additional months. Clinical and radiographic analyses were performed during the study. Implant-tissue samples were prepared for micro computed tomography, backscattered scanning electron microscopy, histomorphometric and histological analyses and ion release measurements. X-ray, SEM and histology images showed that vertical bone resorption in treated implants was lower than in the control group (P<0.05). This effect is likely due to the capacity of the treatments to reduce bacteria colonization on the implant surface. Histological analysis suggested an increase of peri-implant bone formation on silanized implants. However, the short post-ligature period was not enough to detect differences in clinical parameters among implant groups. Within the limits of this study, antibacterial surface treatments have a positive effect against bone resorption induced by peri-implantitis.
本研究的目的是评估抗菌改性牙种植体在种植体周围炎第一阶段的体内效果。在5只比格犬的下颌前磨牙部位植入30颗牙种植体。将种植部位随机分为钛组（未处理的种植体，10个）、钛银组（银电沉积处理，10个）和钛硅烷化组（硅烷化处理，10个）。通过扫描电子显微镜、干涉测量法和X射线光电子能谱对涂层种植体进行表征。种植体植入2个月后，通过结扎引发实验性种植体周围炎。2个月个月个月�个月后移除结扎线，再允许菌斑形成2个月。在研究过程中进行临床和影像学分析。制备种植体组织样本用于微型计算机断层扫描、背散射扫描电子显微镜、组织形态计量学和组织学分析以及离子释放测量。X射线、扫描电子显微镜和组织学图像显示，处理过的种植体的垂直骨吸收低于对照组（P<0.05）。这种效果可能是由于这些处理能够减少种植体表面的细菌定植。组织学分析表明硅烷化种植体周围的骨形成增加。然而，结扎后的短时间不足以检测种植体组之间临床参数的差异。在本研究的范围内，抗菌表面处理对种植体周围炎引起的骨吸收有积极作用。
