Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China; Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Chin J Nat Med. 2018 Jul;16(7):525-545. doi: 10.1016/S1875-5364(18)30089-X.
The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C column (2.1 mm × 100 mm, 1.7 μm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identifled by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim.
本研究旨在鉴定和表征胡桃楸中的主要成分。采用正、负离子模式下的超高效液相色谱-飞行时间质谱联用（UPLC-ESI-Q-TOF/MS）方法，建立并验证了一种简单、高效、灵敏的方法。采用 Waters ACQUITY UPLC BEH C 柱（2.1mm×100mm，1.7μm），以梯度洗脱的方式，用流动相（A 相：0.1%甲酸水溶液，B 相：0.1%甲酸乙腈溶液）进行分离。通过靶向和非靶向峰发现方法，快速筛选出 165 种化合物，然后通过与对照品比较或通过质谱碎片离子分析和文献数据推断，对这些化合物进行了初步鉴定。这些化合物包括 68 种萘醌、20 种二芳基庚烷、29 种类黄酮、20 种三萜和 28 种酚酸。总之，本研究为鉴定胡桃楸等中草药复杂基质中的成分提供了一种有效的方法。
