Department of Biochemistry, Center of Research and Post Graduate Studies, Indian Academy Degree College, Bengaluru, Karnataka, India.
IISc Mathematics Initiative, Indian Institute of Science, Bengaluru, Karnataka, India.
PLoS Negl Trop Dis. 2016 Sep 12;10(9):e0004965. doi: 10.1371/journal.pntd.0004965. eCollection 2016 Sep.
Dengue virus (DENV) is a human pathogen and its etiology has been widely established. There are many interactions between DENV and human proteins that have been reported in literature. However, no publicly accessible resource for efficiently retrieving the information is yet available. In this study, we mined all publicly available dengue-human interactions that have been reported in the literature into a database called DenHunt. We retrieved 682 direct interactions of human proteins with dengue viral components, 382 indirect interactions and 4120 differentially expressed human genes in dengue infected cell lines and patients. We have illustrated the importance of DenHunt by mapping the dengue-human interactions on to the host interactome and observed that the virus targets multiple host functional complexes of important cellular processes such as metabolism, immune system and signaling pathways suggesting a potential role of these interactions in viral pathogenesis. We also observed that 7 percent of the dengue virus interacting human proteins are also associated with other infectious and non-infectious diseases. Finally, the understanding that comes from such analyses could be used to design better strategies to counteract the diseases caused by dengue virus. The whole dataset has been catalogued in a searchable database, called DenHunt (http://proline.biochem.iisc.ernet.in/DenHunt/).
登革热病毒（DENV）是一种人类病原体，其病因已得到广泛证实。文献中已经报道了许多 DENV 与人类蛋白之间的相互作用。然而，目前还没有一个可公开访问的资源来有效地检索这些信息。在本研究中，我们将文献中报道的所有已公开的登革热-人类相互作用挖掘到一个名为 DenHunt 的数据库中。我们检索到了 682 种人类蛋白与登革热病毒成分的直接相互作用、382 种间接相互作用和 4120 种在登革热感染细胞系和患者中差异表达的人类基因。我们通过将登革热-人类相互作用映射到宿主相互作用组上，说明了 DenHunt 的重要性，并观察到该病毒靶向多个宿主功能复合物，这些复合物涉及重要的细胞过程，如代谢、免疫系统和信号通路，这表明这些相互作用可能在病毒发病机制中发挥作用。我们还观察到，7%的与登革热病毒相互作用的人类蛋白也与其他传染性和非传染性疾病有关。最后，从这些分析中获得的认识可以用于设计更好的策略来对抗由登革热病毒引起的疾病。整个数据集已在一个可搜索的数据库中进行了编目，称为 DenHunt（http://proline.biochem.iisc.ernet.in/DenHunt/）。
