Bajaj Abhay, Kumar Anand, Yadav Shivani, Kaur Gurwinder, Bala Monu, Singh Nitin Kumar, Mathan Kumar Rajendran, Manickam Natesan, Mayilraj Shanmugam
Environmental Biotechnology Division, CSIR-Indian Institute of Toxicological Research (IITR), Lucknow, 226 001, India.
Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh, 160 036, India.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5228-5235. doi: 10.1099/ijsem.0.001500. Epub 2016 Sep 11.
The taxonomic position of a Gram-stain negative, non-violaceinpigmented bacterium isolated from an insecticide-contaminated site was characterized by a polyphasic approach. The bacterium was able to grow on three different halogenated compounds namely 1-hlorobutane, 1-hloropropane and 1,2-ichloroethane. As a critical step in the degradation of these haloalkanes, stoichiometric amounts of dechlorination were estimated. Based on selective enrichment method for three months, using a highly contaminated mixed chemical soil, a bacterium was obtained and designated as IITR-71T. Its versatility and novelty led us to further characterize it by polyphasic taxonomy. The 16S rRNA gene sequence (1446 bases) comparison showed highest similarity with those of members of the genus Chromobacterium with the most closely related species to strain IITR-71T being Chromobacterium aquaticum (99.3 %) followed by Chromobacterium haemolyticum (98.6 %) and Chromobacterium piscinae (97.1 %). The major ubiquinone was Q-8. Predominant polar lipids are phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The DNA G+C content of IITR-71T was estimated to be 61.2 mol%. The genotypic and phenotypic distinctiveness of IITR-71T and its phylogenetic relationships indicate that IITR-71T represents a novel species, for which the name Chromobacterium alkanivorans sp. nov. is proposed. The type strain is IITR-71T (=MTCC 11059T=JCM 30068T=KCTC 52433T).
采用多相分类法对从受杀虫剂污染场地分离出的革兰氏染色阴性、不产紫菌素的细菌进行分类地位鉴定。该细菌能够在三种不同的卤代化合物上生长，即1-氯丁烷、1-氯丙烷和1,2-二氯乙烷。作为这些卤代烷烃降解的关键步骤，估算了化学计量的脱氯量。基于在高度污染的混合化学土壤中进行三个月的选择性富集方法，获得了一株细菌并命名为IITR-71T。其多功能性和新颖性促使我们通过多相分类法对其进行进一步鉴定。16S rRNA基因序列（1446个碱基）比较显示，与色杆菌属成员的序列相似度最高，与菌株IITR-71T亲缘关系最密切的物种是水生色杆菌（99.3%），其次是溶血色杆菌（98.6%）和鱼色杆菌（97.1%）。主要泛醌为Q-8。主要极性脂类为磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）和双磷脂酰甘油（DPG）。IITR-71T的DNA G+C含量估计为61.2 mol%。IITR-71T的基因型和表型独特性及其系统发育关系表明，IITR-71T代表一个新物种，为此提出了新名称嗜烷色杆菌（Chromobacterium alkanivorans sp. nov.）。模式菌株为IITR-71T（=MTCC 11059T=JCM 30068T=KCTC 52433T）。
