Rahmani Elham, Samimi Mansooreh, Ebrahimi Faraneh Afshar, Foroozanfard Fatemeh, Ahmadi Shahnaz, Rahimi Maryam, Jamilian Mehri, Aghadavod Esmat, Bahmani Fereshteh, Taghizadeh Mohsen, Memarzadeh Mohammad Reza, Asemi Zatollah
Department of Gynecology and Obstetrics, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Mol Cell Endocrinol. 2017 Jan 5;439:247-255. doi: 10.1016/j.mce.2016.09.008. Epub 2016 Sep 9.
This study was conducted to determine the effects of omega-3 fatty acids and vitamin E co-supplementation on gene expression of lipoprotein(a) (Lp[a]) and oxidized low-density lipoprotein (Ox-LDL), lipid profiles and biomarkers of oxidative stress in women with polycystic ovary syndrome (PCOS). This randomized double-blind, placebo-controlled trial was done on 68 women diagnosed with PCOS according to the Rotterdam criteria aged 18-40 years old. Participants were randomly assigned into two groups to receive either 1000 mg omega-3 fatty acids from flaxseed oil containing 400 mg α-Linolenic acid plus 400 IU vitamin E supplements (n = 34) or placebo (n = 34) for 12 weeks. Lp(a) and Ox-LDL mRNA levels were quantified in peripheral blood mononuclear cells of PCOS women with RT-PCR method. Lipid profiles and biomarkers of oxidative stress were quantified at the beginning of the study and after 12-week intervention. Quantitative results of RT-PCR demonstrated that compared with the placebo, omega-3 fatty acids and vitamin E co-supplementation downregulated expressed levels of Lp(a) mRNA (P < 0.001) and Ox-LDL mRNA (P < 0.001) in peripheral blood mononuclear cells of women with PCOS. In addition, compared to the placebo group, omega-3 fatty acids and vitamin E co-supplementation resulted in a significant decrease in serum triglycerides (-22.1 ± 22.3 vs. +7.7 ± 23.6 mg/dL, P < 0.001), VLDL- (-4.4 ± 4.5 vs. +1.5 ± 4.7 mg/dL, P < 0.001), total- (-20.3 ± 16.6 vs. +12.2 ± 26.1 mg/dL, P < 0.001), LDL- (-16.7 ± 15.3 vs. +11.9 ± 26.1 mg/dL, P < 0.001) and total-/HDL-cholesterol (-0.5 ± 0.6 vs. +0.4 ± 0.8, P < 0.001). There were a significant increase in plasma total antioxidant capacity (+89.4 ± 108.9 vs. +5.9 ± 116.2 mmol/L, P = 0.003) and a significant decrease in malondialdehyde levels (-0.3 ± 0.4 vs. -0.008 ± 0.6 μmol/L, P = 0.01) by combined omega-3 fatty acids and vitamin E intake compared with the placebo group. Overall, omega-3 fatty acids and vitamin E co-supplementation for 12 weeks in PCOS women significantly improved gene expression of Lp(a) and Ox-LDL, lipid profiles and biomarkers of oxidative stress.
本研究旨在确定补充ω-3脂肪酸和维生素E对多囊卵巢综合征（PCOS）女性脂蛋白(a)（Lp[a]）和氧化型低密度脂蛋白（Ox-LDL）的基因表达、血脂谱及氧化应激生物标志物的影响。这项随机双盲、安慰剂对照试验针对68名根据鹿特丹标准诊断为PCOS的18至40岁女性进行。参与者被随机分为两组，一组接受含有400mgα-亚麻酸的1000mg来自亚麻籽油的ω-3脂肪酸加400IU维生素E补充剂（n = 34），另一组接受安慰剂（n = 34），为期12周。采用RT-PCR方法对PCOS女性外周血单核细胞中的Lp(a)和Ox-LDL mRNA水平进行定量。在研究开始时和12周干预后对血脂谱和氧化应激生物标志物进行定量。RT-PCR定量结果表明，与安慰剂相比，补充ω-3脂肪酸和维生素E可下调PCOS女性外周血单核细胞中Lp(a) mRNA（P < 0.001）和Ox-LDL mRNA（P < 0.001）的表达水平。此外，与安慰剂组相比，补充ω-3脂肪酸和维生素E可使血清甘油三酯（-22.1±22.3 vs. +7.7±23.6mg/dL，P < 0.001）、极低密度脂蛋白（-4.4±4.5 vs. +1.5±4.7mg/dL，P < 0.001）、总胆固醇（-20.3±16.6 vs. +12.2±26.1mg/dL，P < 0.001）、低密度脂蛋白（-16.7±15.3 vs. +11.9±26.1mg/dL，P < 0.001）以及总胆固醇/高密度脂蛋白胆固醇（-0.5±0.6 vs. +0.4±0.8，P < 0.001）显著降低。与安慰剂组相比，联合摄入ω-3脂肪酸和维生素E可使血浆总抗氧化能力显著增加（+89.4±108.9 vs. +5.9±116.2mmol/L，P = 0.003），丙二醛水平显著降低（-0.3±0.4 vs. -0.008±0.6μmol/L，P = 0.01）。总体而言，PCOS女性补充ω-3脂肪酸和维生素E共12周可显著改善Lp(a)和Ox-LDL的基因表达、血脂谱及氧化应激生物标志物。
