Nutrigenomic Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona, Spain.
Centre Tecnològic de Nutrició i Salut (CTNS), TECNIO, CEICS, Reus, Spain.
Mol Nutr Food Res. 2017 Feb;61(2). doi: 10.1002/mnfr.201600342. Epub 2016 Nov 3.
Studying the flavanol metabolism is essential to identify bioactive compounds, as beneficial effects of flavanols have been attributed to their metabolic products. However, host-related factors, including pathological conditions, may affect flavanol metabolism and, thus, their bioactivity. This study aims to elucidate whether hypertension affects grape seed flavanol metabolism, influencing their bioactivity in relation to hypertension.
Grape seed flavanols' effect on blood pressure (BP) was studied in spontaneously hypertensive rats (SHR) and healthy Wistar rats 6 h after grape seed extract administration (375 mg/kg). Animals were then sacrificed, and plasma bioavailability and aorta distribution of flavanol metabolites were studied by HPLC-MS/MS in both the groups. Grape seed flavanols were only able to decrease BP in SHR. Plasma total flavanol metabolites showed similar levels, being the difference noticed in specific metabolites' concentrations. Specifically, microbial metabolites showed quantitative and qualitative differences between both health states. Moreover, aorta total concentrations were found decreased in SHR. Interestingly, flavanol microbial metabolites were specifically increased SHR aortas, showing qualitative differences in small phenolic forms.
This study demonstrates important differences in bioactivity and target tissue metabolite levels between healthy and diseased rats, indicating potential metabolites responsible of the anti-hypertensive effect.
研究黄烷醇代谢对于鉴定生物活性化合物至关重要，因为黄烷醇的有益作用归因于其代谢产物。然而，宿主相关因素，包括病理状况，可能会影响黄烷醇代谢，从而影响其生物活性。本研究旨在阐明高血压是否会影响葡萄籽黄烷醇代谢，以及它们与高血压相关的生物活性。
在给予葡萄籽提取物（375 mg/kg）后 6 小时，研究了葡萄籽黄烷醇对自发性高血压大鼠（SHR）和健康 Wistar 大鼠血压的影响。然后处死动物，并通过 HPLC-MS/MS 在两组中研究了黄烷醇代谢物的血浆生物利用度和主动脉分布。葡萄籽黄烷醇仅能降低 SHR 的血压。血浆总黄烷醇代谢物的水平相似，仅注意到特定代谢物浓度的差异。具体而言，微生物代谢物在两种健康状态之间表现出定量和定性差异。此外，还发现 SHR 的主动脉总浓度降低。有趣的是，黄烷醇微生物代谢物特异性增加 SHR 主动脉，显示出小酚类形式的定性差异。
本研究表明，健康和患病大鼠之间在生物活性和靶组织代谢物水平方面存在重要差异，表明可能存在负责抗高血压作用的代谢物。
