Margalef Maria, Iglesias-Carres Lisard, Pons Zara, Bravo Francisca Isabel, Muguerza Begoña, Arola-Arnal Anna
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University, Spain.
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University, Spain; Technological Center of Nutrition and Health (CTNS), TECNIO, CEICS, Reus, Spain.
J Nutr Biochem. 2016 Mar;29:90-6. doi: 10.1016/j.jnutbio.2015.11.007. Epub 2015 Nov 24.
Dietary flavanols produce beneficial health effects; once absorbed, they are recognized as xenobiotics and undergo Phase-II enzymatic detoxification. However, flavanols with a degree of polymerization greater than 2 reach the colon, where they are subjected to microbial metabolism and can be further absorbed and undergo Phase-II reactions. In this sense, flavanols' health-promoting properties are mainly attributed to their metabolic products. Several age-related physiological changes have been evidenced, and it is known that flavanols' bioavailability is affected by internal factors. Therefore, this study aimed to elucidate whether animals of different ages, specifically young and adult rats, exhibit differences in their flavanol metabolism and plasma bioavailability. To accomplish this, an acute dose of a grape seed polyphenol extract was administered to male rats; after 2, 4, 7, 24 and 48 h, flavanols and their Phase-II and microbial metabolites were quantified by HPLC-ESI-MS/MS in plasma. The results indicated important age-related quantitative differences in plasma flavanol metabolites. Interestingly, adult rats presented a remarkable reduction in flavanol absorption and Phase-II flavanol metabolism. Consequently, microbial-derived flavanol metabolism is triggered by higher flavanol affluence in the colonic tract. Furthermore, young rats presented a faster metabolic profile than adult rats. Hence, our results indicate that the physiological bioactivities of flavanols may depend on age.
膳食黄烷醇对健康有益；一旦被吸收，它们会被视为外源性物质并经历II相酶解毒过程。然而，聚合度大于2的黄烷醇会到达结肠，在那里它们会经历微生物代谢，并且可以被进一步吸收并进行II相反应。从这个意义上说，黄烷醇的健康促进特性主要归因于它们的代谢产物。已经证实了一些与年龄相关的生理变化，并且已知黄烷醇的生物利用度会受到内部因素的影响。因此，本研究旨在阐明不同年龄的动物，特别是幼年和成年大鼠，在黄烷醇代谢和血浆生物利用度方面是否存在差异。为了实现这一目标，给雄性大鼠急性注射一剂葡萄籽多酚提取物；在2、4、7、24和48小时后，通过HPLC-ESI-MS/MS对血浆中的黄烷醇及其II相和微生物代谢产物进行定量。结果表明血浆黄烷醇代谢产物存在重要的年龄相关定量差异。有趣的是，成年大鼠的黄烷醇吸收和II相黄烷醇代谢显著降低。因此，结肠中较高的黄烷醇含量会引发微生物衍生的黄烷醇代谢。此外，幼年大鼠的代谢速度比成年大鼠快。因此，我们的结果表明黄烷醇的生理生物活性可能取决于年龄。
