Margalef Maria, Pons Zara, Iglesias-Carres Lisard, Bravo Francisca Isabel, Muguerza Begoña, Arola-Arnal Anna
Nutrigenomic Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona 43007, Spain.
Nutrigenomic Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Tarragona 43007, Spain; Centre Tecnològic de Nutrició i Salut (CTNS), TECNIO, CEICS, Reus 43204, Spain.
Food Chem. 2017 Sep 15;231:287-294. doi: 10.1016/j.foodchem.2017.03.141. Epub 2017 Mar 27.
Flavanols, which exert several health benefits, are metabolized after ingestion. Factors such as the host physiological condition could affect the metabolism and bioavailability of flavanols, influencing their bioactivities. This study aimed to qualitatively evaluate whether a pathological state influenced flavanol plasma bioavailability. Standard and cafeteria (CAF) diet fed rats, a robust model of metabolic syndrome (MeS), were administered 1000mg/kg of flavanol enriched grape seed polyphenol extract (GSPE). Flavanols and their metabolites were quantified by HPLC-MS/MS in plasma before and at 2, 4, 7, 24, and 48h after GSPE ingestion. Results showed that in CAF administered rats the maximum time of plasma flavanol concentration was delayed and these animals presented higher levels of plasma phase-II metabolites as well as altered microbial metabolites. In conclusion, this study demonstrated that MeS pathological state modified flavanol bioavailability, supporting the hypothesis that flavanol metabolism, and therefore flavanol functionality, depend on the organism's state of health.
黄烷醇具有多种健康益处，摄入后会被代谢。宿主生理状况等因素会影响黄烷醇的代谢和生物利用度，进而影响其生物活性。本研究旨在定性评估病理状态是否会影响黄烷醇的血浆生物利用度。给食用标准饮食和自助餐（CAF）饮食的大鼠（一种强大的代谢综合征（MeS）模型）灌胃1000mg/kg富含黄烷醇的葡萄籽多酚提取物（GSPE）。在摄入GSPE之前以及之后的2、4、7、24和48小时，通过HPLC-MS/MS对血浆中的黄烷醇及其代谢产物进行定量分析。结果显示，在给予CAF饮食的大鼠中，血浆黄烷醇浓度达到最大值的时间延迟，并且这些动物的血浆II期代谢产物水平较高，微生物代谢产物也发生了改变。总之，本研究表明MeS病理状态改变了黄烷醇的生物利用度，支持了黄烷醇代谢以及黄烷醇功能取决于机体健康状态这一假说。
