Moazeni-Roodi Abdolkarim, Allameh Abdolamir, Harirchi Iraj, Motiee-Langroudi Maziar, Garajei Ata
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R., Iran.
Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, I.R., Iran.
Pathol Oncol Res. 2017 Apr;23(2):355-360. doi: 10.1007/s12253-016-0114-1. Epub 2016 Sep 15.
The aim of this study was to investigate the relationship between the H-ras and Cox-2 gene expression in tumors from Iranian Oral Squamous Cell Carcinoma (OSCC) patients. Fresh tumor biopsies removed from oral cavity were collected from 67 new cases. Total RNA was extracted from biopsies and processed for quantification of H-ras and Cox-2 specific RNA expression using real-time PCR (QPCR). In addition, 59 gingival biopsies from apparently normal individuals were processed for QPCR assays. The results showed that Cox-2 expression at mRNA levels was at minimal levels in normal gingival biopsies. However, there was a surge in Cox-2 expression in tumor tissues (11.5 fold, p < 0.0001). Cox-2 expression was elevated depending on the tumor grade and there was a 1.7 fold increase (p = 0.003) in tumors diagnosed as MD/PD compared to that pathologically diagnosed as WD. This inflammatory marker was increased more significantly in smoker patients compared to non-smoker matching group. The H-ras expression at mRNA levels was significantly higher in OSCC samples compared to normal gingival (3 fold; p = 0.044). This expression was significantly higher in tumors diagnosed as MD/PD compared to WD (1.59 fold, p = 0.033). In conclusion, we found a correlation between H-ras expression and Cox-2 induction in OSCC tissue, suggesting that together these genes are contributing to cancer progression. Cox-2 is an early event in cancers of mucosal epithelial cells and a surge in Cox-2 expression in OSCC could be partly due to pro-inflammatory factors such as smoking.
本研究旨在调查伊朗口腔鳞状细胞癌（OSCC）患者肿瘤中H-ras与Cox-2基因表达之间的关系。从67例新病例中收集了从口腔切除的新鲜肿瘤活检组织。从活检组织中提取总RNA，并使用实时荧光定量PCR（QPCR）对H-ras和Cox-2特异性RNA表达进行定量分析。此外，对59例明显正常个体的牙龈活检组织进行QPCR检测。结果显示，正常牙龈活检组织中Cox-2 mRNA水平的表达处于最低水平。然而，肿瘤组织中Cox-2表达激增（11.5倍，p<0.0001）。Cox-2表达根据肿瘤分级升高，与病理诊断为高分化（WD）的肿瘤相比，诊断为中/低分化（MD/PD）的肿瘤中Cox-2表达增加了1.7倍（p=0.003）。与非吸烟匹配组相比，吸烟患者中这种炎症标志物的增加更为显著。与正常牙龈相比，OSCC样本中H-ras mRNA水平的表达显著更高（3倍；p=0.044）。与高分化肿瘤相比，中/低分化肿瘤中这种表达显著更高（1.59倍，p=0.033）。总之，我们发现OSCC组织中H-ras表达与Cox-2诱导之间存在相关性，表明这些基因共同促进癌症进展。Cox-2是黏膜上皮细胞癌的早期事件，OSCC中Cox-2表达的激增可能部分归因于吸烟等促炎因素。
