Barroqueiro Elizabeth S B, Prado Dayanna S, Barcellos Priscila S, Silva Tonicley A, Pereira Wanderson S, Silva Lucilene A, Maciel Márcia C G, Barroqueiro Rodrigo B, Nascimento Flávia R F, Gonçalves Azizedite G, Guerra Rosane N M
Centro de Ciências Biológicas e da Saúde, Laboratorio de Imunofisiologia, Universidade Federal do Maranhão, Cidade Univesitária Dom Delgado, No. 1966, Avenida dos Portugueses, 6080-580 São Luís, MA, Brazil.
Evid Based Complement Alternat Med. 2016;2016:2859652. doi: 10.1155/2016/2859652. Epub 2016 Aug 18.
Attalea speciosa syn Orbignya phalerata Mart. (babassu) has been used in the treatment of inflammatory and infectious diseases. Aim of the study. To investigate the antimicrobial and immunological activity of babassu mesocarp extract (EE). Material and Methods. The in vitro antimicrobial activity was evaluated by disk diffusion assay and by determination of the minimum inhibitory concentration (MIC) to Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA). The flavonoids and phenolic acids content were determined by chromatography. The in vivo assays were performed in Swiss mice submitted to sepsis by cecal ligation and puncture (CLP). The mice received EE subcutaneously (125 or 250 mg/Kg), 6 hours after the CLP. The number of lymphoid cells was quantified and the cytokines production was determined by ELISA after 12 h. Results. EE was effective as antimicrobial to E. faecalis, S. aureus, and MRSA. EE is rich in phenolic acids, a class of compounds with antimicrobial and immunological activity. An increased survival can be observed in those groups, possibly due to a significant inhibition of TNF-α and IL-6. Conclusions. The EE showed specific antimicrobial activity in vitro and an important antiseptic effect in vivo possibly due to the antimicrobial and immunomodulatory activity.
阿塔莱棕（学名：Attalea speciosa，异名：Orbignya phalerata Mart.）（巴巴苏棕）已被用于治疗炎症和传染病。研究目的。研究巴巴苏中果皮提取物（EE）的抗菌和免疫活性。材料与方法。通过纸片扩散法以及测定对大肠杆菌、铜绿假单胞菌、粪肠球菌、金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的最低抑菌浓度（MIC）来评估体外抗菌活性。通过色谱法测定黄酮类化合物和酚酸的含量。体内试验在通过盲肠结扎和穿刺（CLP）诱导脓毒症的瑞士小鼠中进行。小鼠在CLP后6小时皮下注射EE（125或250mg/Kg）。12小时后通过ELISA测定淋巴细胞数量并确定细胞因子的产生。结果。EE对粪肠球菌、金黄色葡萄球菌和MRSA具有抗菌作用。EE富含酚酸，这是一类具有抗菌和免疫活性的化合物。在这些组中可观察到存活率提高，可能是由于对TNF-α和IL-6的显著抑制。结论。EE在体外显示出特定的抗菌活性，在体内具有重要的防腐作用，这可能归因于其抗菌和免疫调节活性。
