Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Biosens Bioelectron. 2017 Jan 15;87:679-685. doi: 10.1016/j.bios.2016.08.087. Epub 2016 Aug 27.
We described a novel resonance light scattering (RLS) sensor for the specific recognition of trace quantities of Hepatitis A Virus (HAV); the sensor was based on a mussel-inspired hepatitis molecularly imprinted polymer. As a recognition element, polydopamine (PDA)-coated totivirus-imprinted polymer was introduced on the surface of SiO nanoparticles (virus-imprinted SiO@PDA NPs) using an efficient one-step synthesis method. The target virus was selectively captured by the imprinted polymer films, thereby increasing the RLS intensity. A simple fluorescence spectrophotometer was employed to measure the changes in the intensity. The enhanced RLS intensity (∆I) was proportional to the concentration of HAV in the range of 0.04-6.0nmol∙L, with a low limit of detection of 8.6pmol∙L. The selectivity study confirmed that the resultant HAV-imprinted SiO@PDA NPs possessed high selectivity for HAV. The sensor was successfully applied for the direct detection of additional HAV from a 20,000-fold dilution of human serum. The proposed strategy is simple, eco-friendly, highly selective, and sensitive.
我们描述了一种新型的共振光散射（RLS）传感器，用于特异性识别痕量甲型肝炎病毒（HAV）；该传感器基于贻贝启发的肝炎分子印迹聚合物。作为识别元件，使用高效的一步合成方法将聚多巴胺（PDA）涂层的 totivirus 印迹聚合物引入到 SiO 纳米粒子（印迹 SiO@PDA NPs）的表面。目标病毒被印迹聚合物膜选择性捕获，从而增加了 RLS 强度。简单的荧光分光光度计用于测量强度的变化。增强的 RLS 强度（∆I）与 HAV 的浓度在 0.04-6.0nmol·L 范围内呈正比，检测下限低至 8.6pmol·L。选择性研究证实，所得的 HAV 印迹 SiO@PDA NPs 对 HAV 具有高选择性。该传感器成功地用于直接检测来自人血清 20,000 倍稀释的额外 HAV。所提出的策略简单、环保、高选择性和灵敏。
