Chair of Analytical Food Chemistry, Technische Universität München , Alte Akademie 10, 85354 Freising, Germany.
J Agric Food Chem. 2016 Sep 28;64(38):7259-64. doi: 10.1021/acs.jafc.6b02913. Epub 2016 Sep 15.
A toxicokinetic study of the Fusarium mycotoxins enniatin B1 (ENN B1) and enniatin B (ENN B) was performed in broiler chickens. Each animal received ENN B1 or B orally via an intracrop bolus and intravenously at a dose of 0.2 mg/kg body weight. Both enniatins were poorly absorbed after oral administration, with absolute oral bioavailabilities of 0.05 and 0.11 for ENNs B1 and B, respectively. Both enniatins were readily distributed to the tissues, with mean volumes of distribution of 25.09 and 33.91 L/kg for ENNs B1 and B, respectively. The mean total body clearance was rather high, namely, 6.63 and 7.10 L/h/kg for ENNs B1 and B, respectively. Finally, an UHPLC-HRMS targeted approach was used to investigate the phase I and II biotransformations of both mycotoxins. Oxygenation was the major phase I biotransformation pathway for both ENNs B1 and B. Neither glucuronide nor sulfate phase II metabolites were detected.
在肉鸡中进行了镰刀菌真菌毒素恩镰菌素 B1（ENN B1）和恩镰菌素 B（ENN B）的毒代动力学研究。每个动物通过直肠内推注和静脉内以 0.2 毫克/公斤体重的剂量分别口服接受 ENN B1 或 B。口服后，两种恩镰菌素的吸收都很差，ENN B1 和 B 的绝对口服生物利用度分别为 0.05 和 0.11。两种恩镰菌素都容易分布到组织中，ENN B1 和 B 的平均分布体积分别为 25.09 和 33.91 L/kg。总的清除率相当高，ENN B1 和 B 的平均总清除率分别为 6.63 和 7.10 L/h/kg。最后，采用 UHPLC-HRMS 靶向方法研究了这两种真菌毒素的 I 期和 II 期生物转化。氧合是两种 ENN B1 和 B 的主要 I 期生物转化途径。未检测到葡萄糖醛酸或硫酸盐的 II 期代谢物。
