Abo-Elfadl Mahmoud T, Gamal-Eldeen Amira M, Elshafey Mostafa M, Abdalla Gamil M, Ali Shawkey S, Ali Moustafa R K, Zawrah Mahmoud F M
Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Department of Biochemistry, National Research Centre, Cairo, Egypt.
Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Department of Biochemistry, National Research Centre, Cairo, Egypt.
J Photochem Photobiol B. 2016 Nov;164:21-29. doi: 10.1016/j.jphotobiol.2016.09.012. Epub 2016 Sep 13.
The photothermal properties of gold nanoparticles (GNPs) are promising therapeutic modality for cancer. The study objective is to evaluate the therapeutic effect of the prepared PEGylated gold nano-semicubes (PEG-GNSCs) in skin cancer. The synthesized PEG-GNSCs were intermediate between cubic and rod shapes (low aspect ratio- rods).
In vitro toxicity was investigated in human skin melanoma Sk-Mel-28 cells, and skin squamous cell carcinoma was induced in CD1 mice by dimethylbenzanthracene (DMBA) and 12-O-tetradecanoyl-phorbol-13-acetate (TPA).
The calculated IC in Sk-Mel-28 cells was 3.41μg/ml of PEG-GNSCs, in presence of laser exposure. Photothermal therapy using laser-stimulated PEG-GNSCs resulted in inhibited volume of skin tumors. Our findings indicated that the inflammatory mediators, nitric oxide and cycloxygenase-2, were inhibited in mice after being treated with low and high doses of PEG-GNSCs, accompanied with laser exposure. However, the tumor necrosis factor -α was markedly elevated, while there was no change in 5-lipoxygenase. The pro-angiogenic factor vascular endothelial growth factor was inhibited, while histone acetylation and apoptosis were induced in tumor-bearing groups, after being treated with laser-stimulated PEG-GNSCs.
The present study indicated the promising photothermal therapeutic effect of laser-stimulated PEG-GNSCs as an effective modality to inhibit the tumor growth, the angiogenesis and partially the inflammation.
金纳米颗粒（GNPs）的光热特性是一种很有前景的癌症治疗方式。本研究的目的是评估制备的聚乙二醇化金纳米半立方体（PEG-GNSCs）对皮肤癌的治疗效果。合成的PEG-GNSCs呈立方和棒状之间的形态（低纵横比棒状）。
在人皮肤黑色素瘤Sk-Mel-28细胞中研究体外毒性，并通过二甲基苯并蒽（DMBA）和12-O-十四烷酰佛波醇-13-乙酸酯（TPA）在CD1小鼠中诱导皮肤鳞状细胞癌。
在激光照射下，Sk-Mel-28细胞中计算得出的PEG-GNSCs的半数抑制浓度（IC）为3.41μg/ml。使用激光刺激的PEG-GNSCs进行光热疗法可使皮肤肿瘤体积受到抑制。我们的研究结果表明，用低剂量和高剂量的PEG-GNSCs并伴随激光照射处理后的小鼠，炎症介质一氧化氮和环氧化酶-2受到抑制。然而，肿瘤坏死因子-α明显升高，而5-脂氧合酶没有变化。在用激光刺激的PEG-GNSCs处理后，促血管生成因子血管内皮生长因子受到抑制，而在荷瘤组中诱导了组蛋白乙酰化和细胞凋亡。
本研究表明激光刺激的PEG-GNSCs具有良好的光热治疗效果，是抑制肿瘤生长、血管生成以及部分炎症的有效方式。
