F Rahimi-Moghaddam, N Sattarahmady, N Azarpira
Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Biomed Phys Eng. 2019 Aug 1;9(4):473-482. doi: 10.31661/jbpe.v0i0.906. eCollection 2019 Aug.
Au nanoparticles (AuNPs) exhibit very unique physiochemical and optical properties, which now are extensively studied in a range of medical diagnostic and therapeutic applications. AuNPs can be used for cancer clinical treatment with minimal invasion. On the other hand, curcumin is a polyphenol derived from turmeric which is used for medical purposes due to its anti-cancer, anti-microbial, anti-oxidant and anti-inflammatory properties. Despite these potential properties of curcumin, its usage is limited in medicine due to low solubility in water. Conjugation of curcumin to AuNPs (Au-Cur nanostructure) can be increasing its solubility. Photo-thermal therapy (PTT) is a novel kind of cancer treatment which involves two major components: laser and photo-thermoconversion agents.
Here, diode lasers emitting 808 nm and 650 nm were utilized as light sources, and synthesized Au-Cur nanostructure was applied as a photo-thermo conversion agent. UV-vis absorbance spectroscopy and dynamic light scattering (DLS) were applied to study the maximum absorption of particles, size stability of the samples and their zeta potential. The synthesized Au-Cur nanostructure under irradiation of laser is used for PPT on 4T1 cells. The cytotoxicity activity of Au-Cur nanostructure and laser irradiation on 4T1 cells was evaluated by MTT assay.
Synthesized Au-Cur nanostructure showed λ at 540 nm and a mean hydrodynamic diameter of 25.8 nm. 4T1 cells were exposed to an 808 nm diode laser (1.5 W cm, 10 min) in the presence of different concentrations of Au-Cur nanostructure. Next, 4T1 cells with Au-Vur nanostructure were exposed to diode laser beam (650 nm, 1.5 W cm) for 10 min. The results revealed that Au-Cur nanostructure under laser irradiation of 808 nm more decreased cell viability of 4T1 cells compared to laser irradiation of 650 nm.
It was concluded that combining an 808-nm laser at a power density of 1.5W/cm with Au-Cur nanostructure has a destruction effect on 4T1 breast cancer cells in vitro experiments compared to laser irradiation of 650 nm.
金纳米颗粒（AuNPs）具有非常独特的物理化学和光学性质，目前在一系列医学诊断和治疗应用中得到广泛研究。AuNPs可用于癌症的微创临床治疗。另一方面，姜黄素是一种从姜黄中提取的多酚，由于其抗癌、抗菌、抗氧化和抗炎特性而被用于医学目的。尽管姜黄素有这些潜在特性，但其在医学上的应用因在水中溶解度低而受到限制。姜黄素与AuNPs共轭（Au-Cur纳米结构）可提高其溶解度。光热疗法（PTT）是一种新型的癌症治疗方法，涉及两个主要成分：激光和光热转换剂。
在此，使用发射808nm和650nm的二极管激光器作为光源，并将合成的Au-Cur纳米结构用作光热转换剂。采用紫外可见吸收光谱和动态光散射（DLS）研究颗粒的最大吸收、样品的尺寸稳定性及其zeta电位。合成的Au-Cur纳米结构在激光照射下用于对4T1细胞进行光热疗法。通过MTT法评估Au-Cur纳米结构和激光照射对4T1细胞的细胞毒性活性。
合成的Au-Cur纳米结构在540nm处显示出λ，平均流体动力学直径为25.8nm。在不同浓度的Au-Cur纳米结构存在下，4T1细胞暴露于808nm二极管激光器（1.5W/cm，10分钟）。接下来，将具有Au-Vur纳米结构的4T1细胞暴露于二极管激光束（650nm，1.5W/cm）10分钟。结果显示，与650nm激光照射相比，808nm激光照射下的Au-Cur纳米结构对4T1细胞活力的降低作用更大。
得出的结论是，在体外实验中，与650nm激光照射相比，功率密度为1.5W/cm的808nm激光与Au-Cur纳米结构相结合对4T1乳腺癌细胞具有破坏作用。
