Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Sci Rep. 2016 Sep 20;6:33580. doi: 10.1038/srep33580.
B cells contribute to the development of dilated cardiomyopathy (DCM) by inducing myocyte injuries and myocardial fibrosis. Our recent research indicated that microRNA (miR) -185 participated in human B-cell activation. Thus, this study was aimed to explore the relationship between miR-185 and DCM progression. Forty-one healthy volunteers and fifty newly diagnosed DCM patients were enrolled. The levels of plasma miR-185, TNF-α secreting B cells, and anti-heart autoantibody were detected. We found that the mean levels of plasma miR-185 in DCM patients were significantly higher than those in healthy controls. Furthermore, these DCM patients could be divided into miR-185(high) and miR-185(low) groups according to the cluster distribution. During one-year follow-up period, the miR-185(high) group showed apparent improvements in left ventricular ejection fraction, left ventricular end diastolic diameter, and NT-proBNP, accompanied by significant declines in both cardiovascular mortality and total admissions for heart failure re-hospitalizations. In addition, the levels of anti-β1-AR antibody and TNF-α secreting B cells were also reduced in miR-185(high) group. These findings suggested that high miR-185 levels might be associated with a favorable prognosis by repressing B cell function in DCM. The findings of this study need to be confirmed with larger sample size and longer duration of observation.
B 细胞通过诱导心肌损伤和心肌纤维化参与扩张型心肌病（DCM）的发展。我们最近的研究表明，微小 RNA（miR）-185 参与了人类 B 细胞的激活。因此，本研究旨在探讨 miR-185 与 DCM 进展的关系。纳入了 41 名健康志愿者和 50 名新诊断的 DCM 患者。检测了血浆 miR-185、分泌 TNF-α的 B 细胞和抗心脏自身抗体的水平。我们发现，DCM 患者的血浆 miR-185 水平明显高于健康对照组。此外，根据聚类分布，这些 DCM 患者可分为 miR-185（高）和 miR-185（低）组。在为期一年的随访期间，miR-185（高）组的左心室射血分数、左心室舒张末期直径和 NT-proBNP 明显改善，同时心血管死亡率和心力衰竭再入院的总入院率也显著下降。此外，miR-185（高）组的抗β1-AR 抗体和分泌 TNF-α的 B 细胞水平也降低了。这些发现表明，高 miR-185 水平可能通过抑制 DCM 中的 B 细胞功能与良好的预后相关。这项研究的发现需要更大的样本量和更长的观察时间来证实。
