Busse H, El-Banna T, Auling G
Institut für Mikrobiologie der Universität, Hannover, Federal Republic of Germany.
Appl Environ Microbiol. 1989 Jun;55(6):1578-83. doi: 10.1128/aem.55.6.1578-1583.1989.
Different approaches were evaluated to identify aerobic, gram-negative, biodegradative isolates assumed to be pseudomonads. Quinone and polyamine analysis allowed rapid identification to the genus level, i.e., allocation of those isolates belonging to the Pseudomonas fluorescens complex which represents the phylogenetically defined core of the heterogeneous genus Pseudomonas. Subsequent studies concentrated only on these true pseudomonads. The multiple-test system API 20NE, determination of the moles percent G+C content, and polyacrylamide gel electrophoresis of soluble proteins aided in identification on the species level. Polyacrylamide gel electrophoresis of both soluble proteins and whole-cell lipopolysaccharides allowed recognition of identical strains and double isolates, which were confirmed by DNA-DNA hybridization.
评估了不同方法以鉴定假定为假单胞菌的需氧、革兰氏阴性、可生物降解分离株。醌和多胺分析可快速鉴定到属水平，即对那些属于荧光假单胞菌复合体的分离株进行分类，该复合体代表了异质假单胞菌属系统发育定义的核心。后续研究仅集中在这些真正的假单胞菌上。多重测试系统API 20NE、G+C摩尔百分比含量的测定以及可溶性蛋白质的聚丙烯酰胺凝胶电泳有助于在种水平上进行鉴定。可溶性蛋白质和全细胞脂多糖的聚丙烯酰胺凝胶电泳可识别相同菌株和双分离株，这通过DNA-DNA杂交得到了证实。
