多胺在产甲烷菌中的分布
Distribution of polyamines in methanogenic bacteria.
作者信息
Scherer P, Kneifel H
出版信息
J Bacteriol. 1983 Jun;154(3):1315-22. doi: 10.1128/jb.154.3.1315-1322.1983.
Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentrations; members of Methanococcaceae (family II) were characterized by putrescine and high spermidine concentrations; members of Methanomicrobiaceae (family III) were characterized by the presence of putrescine, spermidine, and sym-homospermidine; and members of Methanosarcinaceae (family IV) contained only high concentrations of sym-homospermidine in addition to putrescine. The highest polyamine concentration was found in Methanosarcina barkeri Jülich, with 0.35% putrescine in the dry cell material. The polyamine distribution found coincides with the dendrogram based on comparative cataloguing of 16S rRNA and offers a new, rapid chemotaxonomic method for characterizing methanogenic bacteria. Variation of the growth substrates (H2-CO2, methanol, acetate, and trimethylamine) for M. barkeri resulted in quantitative but not qualitative differences in polyamine composition.
对所有四个产甲烷菌科的成员进行了多胺浓度分析。对丹磺酰化细胞提取物的高效液相色谱分析揭示了每个科典型的多胺模式。甲烷杆菌科（第一科）的成员以极低的多胺浓度为特征；甲烷球菌科（第二科）的成员以腐胺和高浓度亚精胺为特征；甲烷微菌科（第三科）的成员以存在腐胺、亚精胺和对称高亚精胺为特征；甲烷八叠球菌科（第四科）的成员除腐胺外仅含有高浓度的对称高亚精胺。在巴氏甲烷八叠球菌尤利希菌株中发现了最高的多胺浓度，干细胞物质中腐胺含量为0.35%。所发现的多胺分布与基于16S rRNA比较编目的树状图一致，并提供了一种新的、快速的化学分类方法来表征产甲烷菌。巴氏甲烷八叠球菌生长底物（H2-CO2、甲醇、乙酸盐和三甲胺）的变化导致多胺组成在数量上而非质量上的差异。
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