Complete nucleotide sequence and characterization of a cryptic plasmid from Lactobacillus helveticus subsp. jugurti.
作者信息
Takiguchi R, Hashiba H, Aoyama K, Ishii S
机构信息
Technical Research Institute, Snow Brand Products Co., Ltd., Saitama, Japan.
出版信息
Appl Environ Microbiol. 1989 Jun;55(6):1653-5. doi: 10.1128/aem.55.6.1653-1655.1989.
Abstract
A small cryptic plasmid, pLJ1, was isolated from Lactobacillus helveticus subsp. jugurti and was cloned into Escherichia coli HB101 by using pBR329 as a vector. Plasmid pLJ1 was 3,292 base pairs long and had single restriction endonuclease sites for PvuII, KpnI, AvaII, Acci, HindIII, and EcoRI. In a maxicell system, pLJ1 produced a protein of about 41 kilodaltons.
摘要
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