巴西亚马逊海岸水鸟中禽流感病毒的监测
Surveillance of Avian Influenza Virus in Aquatic Birds on the Brazilian Amazon Coast.
作者信息
Hurtado Renata, de Azevedo-Júnior Severino Mendes, Vanstreels Ralph Eric Thijl, Fabrizio Thomas, Walker David, Rodrigues Roberta C, Seixas Marina M M, de Araújo Jansen, Thomazelli Luciano M, Ometto Tatiana Lopes, Webby Richard J, Webster Robert G, Jerez José Antonio, Durigon Edison Luiz
机构信息
Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
出版信息
Ecohealth. 2016 Dec;13(4):813-818. doi: 10.1007/s10393-016-1169-x. Epub 2016 Sep 19.
The occurrence of avian influenza viruses (AIV) has been extensively studied in aquatic birds in the Northern hemisphere; however, much less information is available for the South American region. In 2009-2010, we sampled 1006 wild aquatic birds (90% Charadriiformes, 9% Anseriformes, and 1% other groups) at three locations on the Brazilian Amazon coast, a region that serves as a major stop-over and wintering site along the Atlantic Americas flyway. Real-time RT-PCR identified five samples as positive; however, no AIV isolates could be obtained and Illumina sequencing did not produce gene sequences that would allow further characterization of the virus.
禽流感病毒（AIV）在北半球水鸟中的发生情况已得到广泛研究；然而，关于南美地区的信息却少得多。在2009年至2010年期间，我们在巴西亚马逊海岸的三个地点对1006只野生水鸟（90%为鸻形目，9%为雁形目，1%为其他类群）进行了采样，该地区是沿大西洋美洲飞行路线的一个主要中途停留和越冬地点。实时逆转录聚合酶链反应（RT-PCR）鉴定出五个样本呈阳性；然而，未能获得AIV分离株，且Illumina测序未产生可用于病毒进一步特征分析的基因序列。
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