Wankhede Lachi, Price Bridget Signe, Osorio-González Carlos Saul, Saini Rahul, Brar Satinder Kaur
Department of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, ON, M3J 1P3, Canada.
School of Chemical Biological and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, USA.
Bioprocess Biosyst Eng. 2025 Jul 19. doi: 10.1007/s00449-025-03209-2.
The shift toward sustainable biofuels and bioproducts has increased interest in microbial production systems using renewable substrates. This study explores the use of wood hydrolysate, an abundant, cost-effective lignocellulosic substrate, as the primary carbon source for lipid and carotenoid production by Rhodosporidium toruloides-7191 under fed-batch cultivation in a 3-L bioreactor. The fed-batch strategy, chosen over batch and continuous modes, enables controlled nutrient supply, minimizes substrate inhibition, and maintains a favorable carbon-to-nitrogen ratio, thereby supporting prolonged biosynthesis and higher product yields. The process achieved a maximum lipid production of 22.33 g/L, a total lipid accumulation of 57.9% and a total carotenoid production of 4.23 mg/L. Fatty acid analysis shows a composition rich in linoleic acid (C18:2), oleic acid (C18:1), and palmitic acid (C16:0), indicating its suitability for biodiesel applications. The results emphasize R. toruloides-7191 as a promising candidate for industrial-scale applications, advancing sustainable production of biofuels and high-value bioproducts. The effectiveness of wood hydrolysate as a substrate further supports the feasibility of this approach, highlighting its potential in advancing industrial-scale processes for the production of biofuels and value-added compounds.
向可持续生物燃料和生物产品的转变增加了人们对使用可再生底物的微生物生产系统的兴趣。本研究探索了使用木材水解物这种丰富且经济高效的木质纤维素底物，作为在3升生物反应器中补料分批培养条件下，红酵母Rhodosporidium toruloides - 7191生产脂质和类胡萝卜素的主要碳源。选择补料分批策略而非分批和连续模式，能够实现营养物质的可控供应，将底物抑制降至最低，并维持有利的碳氮比，从而支持延长生物合成过程并提高产品产量。该过程实现了最大脂质产量为22.33克/升，总脂质积累量为57.9%，总类胡萝卜素产量为4.23毫克/升。脂肪酸分析表明其成分富含亚油酸（C18:2）、油酸（C18:1）和棕榈酸（C16:0），表明其适用于生物柴油应用。结果强调了Rhodosporidium toruloides - 7191作为工业规模应用的有前景候选者，推动了生物燃料和高价值生物产品的可持续生产。木材水解物作为底物的有效性进一步支持了这种方法的可行性，突出了其在推进生物燃料和增值化合物生产的工业规模过程中的潜力。
