Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China. Institute for Interdisciplinary Research & Key Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, Jianghan University, Wuhan 430056, People's Republic of China.
Nanotechnology. 2016 Oct 28;27(43):435102. doi: 10.1088/0957-4484/27/43/435102. Epub 2016 Sep 22.
A colorimetric platform for influenza A virus detection was developed by using the high efficiency of enzymatic catalysis and the reduction of gold ions with hydrogen peroxide. Aptamer-functionalized magnetic microparticles were synthesized to capture the influenza A virus. This was followed by the binding of ConA-GOx-AuNPs to the H3N2 virus through the ConA-glycan interaction. The sandwich complex was subsequently dispersed in glucose solution to trigger an enzymatic reaction to produce hydrogen peroxide, which controlled the growth of gold nanoparticles and produced colored solutions. The determination of H3N2 concentration was realized by comparing the two differently colored gold nanoparticles. This method could detect the target virus as low as 11.16 μg ml(-1). Furthermore, it opens new opportunities for sensitive and colorimetric detection of viruses and proteins.
本研究开发了一种基于酶催化效率和过氧化物还原金离子的比色法流感 A 病毒检测平台。通过将适配体功能化的磁性微球用于捕获流感 A 病毒，然后通过 ConA-糖基相互作用将 ConA-GOx-AuNPs 结合到 H3N2 病毒上。所得夹心复合物随后在葡萄糖溶液中分散，以引发酶反应产生过氧化氢，从而控制金纳米粒子的生长并产生有色溶液。通过比较两种不同颜色的金纳米粒子来实现对 H3N2 浓度的测定。该方法可检测到低至 11.16 μg ml(-1) 的目标病毒。此外，该方法为病毒和蛋白质的灵敏比色检测开辟了新的机会。
