生长停滞和DNA损伤诱导蛋白45β（GADD45β）是一种抗肿瘤基因，可抑制鸡的J亚群禽白血病病毒复制。

GADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens.

作者信息

Zhang Xinheng, Yan Zhuanqiang, Li Xinjian, Lin Wencheng, Dai Zhenkai, Yan Yiming, Lu Piaopiao, Chen Weiguo, Zhang Huanmin, Chen Feng, Ma Jingyun, Xie Qingmei

机构信息

College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.

Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.

出版信息

Oncotarget. 2016 Oct 18;7(42):68883-68893. doi: 10.18632/oncotarget.12027.

Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45β inhibited ALV-J replication. GADD45β could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.

禽白血病病毒J亚群（ALV-J）是一种逆转录病毒，可导致鸡发生肿瘤、肝肿大、免疫抑制和生产性能下降。ALV-J的致瘤和致病机制仍然是一个热门话题。为了探索促进鸡对ALV-J感染产生抗性的抗肿瘤基因，我们培育了抗ALV-J和易感ALV-J的鸡（F3代）。对来自抗ALV-J和易感ALV-J鸡的肝脏组织进行RNA测序（RNA-Seq），鉴定出216个差异表达基因；其中88个基因在抗ALV-J鸡中上调（与易感鸡相比）。我们根据这些基因参与的生物信号通路，筛选出抗ALV-J鸡中显著上调（P < 0.01）的感兴趣基因。功能分析表明，GADD45β的过表达抑制了ALV-J的复制。GADD45β可以增强对ALV-J感染的防御能力，可作为鉴定ALV-J感染的分子标记。