School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University , Wenzhou 325027, China.
Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , Wenzhou 32500, China.
ACS Appl Mater Interfaces. 2016 Oct 12;8(40):27207-27217. doi: 10.1021/acsami.6b08893. Epub 2016 Sep 30.
The adherence of bacteria and the formation of biofilm on implants is a serious problem that often leads to implant failure. A series of antimicrobial coatings have been constructed to resist bacterial adherence or to kill bacteria through contact with or release of antibacterial agents. The accumulation of dead bacteria facilitates further bacterial contamination and biofilm development. Herein, we have designed and constructed a novel, reversibly switchable bactericidal and antifouling surface through surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization to combine thermally responsive N-isopropylacrylamide (NIPAAm) and bactericidal quaternary ammonium salts (2-(dimethylamino)-ethyl methacrylate (DMAEMA)). Measurements of spectroscopic ellipsometry and water contact angle and X-ray photoelectron spectroscopy were used to examine the process of the surface functionalization. The temperature-responsive P(DMAEMA-co-NIPAAm) copolymer coating can switch by phase transition between a hydrophobic capturing surface at high temperatures and a relatively hydrophilic antifouling surface at lower temperatures. The quaternary ammonium salts of PDMAEMA displayed bactericidal efficiency against both Escherichia coli and Staphylococcus aureus. The functionalized surface could efficiently prevent bovine serum albumin adsorption and had good biocompatibility against human lens epithelial cells.
细菌黏附和生物膜在植入物上的形成是一个严重的问题，往往会导致植入物失效。已经构建了一系列抗菌涂层来抵抗细菌黏附，或通过接触或释放抗菌剂来杀死细菌。死细菌的积累会促进进一步的细菌污染和生物膜的发展。在此，我们通过表面引发可逆加成-断裂链转移（RAFT）聚合设计并构建了一种新颖的、可反复开关的杀菌和抗污表面，将热敏性 N-异丙基丙烯酰胺（NIPAAm）和杀菌季铵盐（2-（二甲氨基）-乙基甲基丙烯酸酯（DMAEMA））结合在一起。光谱椭圆光度法、水接触角和 X 射线光电子能谱测量用于检测表面功能化过程。温度响应性 P(DMAEMA-co-NIPAAm)共聚物涂层可以通过在高温下的疏水性捕获表面和在较低温度下的相对亲水性抗污表面之间的相变进行切换。PDMAEMA 的季铵盐对大肠杆菌和金黄色葡萄球菌均显示出杀菌效率。功能化表面可以有效地防止牛血清白蛋白的吸附，并且对人晶状体上皮细胞具有良好的生物相容性。
