• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

IMGT/StatClonotype用于对来自IMGT/HighV-QUEST的NGS免疫球蛋白（IG）和T细胞受体（TR）IMGT克隆型（氨基酸序列）多样性或表达进行成对评估和可视化。

IMGT/StatClonotype for Pairwise Evaluation and Visualization of NGS IG and TR IMGT Clonotype (AA) Diversity or Expression from IMGT/HighV-QUEST.

作者信息

Aouinti Safa, Giudicelli Véronique, Duroux Patrice, Malouche Dhafer, Kossida Sofia, Lefranc Marie-Paule

机构信息

IMGT®, The international ImMunoGeneTics information system®, Laboratoire d'ImmunoGénétique Moléculaire LIGM, Institut de Génétique Humaine IGH, UPR CNRS 1142, Montpellier University, Montpellier, France; Higher School of Statistics and Information Analysis, Unité Modélisation et Analyse Statistique et Economique, University of Carthage, Tunis, Tunisia.

IMGT®, The international ImMunoGeneTics information system®, Laboratoire d'ImmunoGénétique Moléculaire LIGM, Institut de Génétique Humaine IGH, UPR CNRS 1142, Montpellier University , Montpellier , France.

出版信息

Front Immunol. 2016 Sep 9;7:339. doi: 10.3389/fimmu.2016.00339. eCollection 2016.

DOI:10.3389/fimmu.2016.00339
PMID:27667992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5016520/
Abstract

There is a huge need for standardized analysis and statistical procedures in order to compare the complex immune repertoires of antigen receptors immunoglobulins (IG) and T cell receptors (TR) obtained by next generation sequencing (NGS). NGS technologies generate millions of nucleotide sequences and have led to the development of new tools. The IMGT/HighV-QUEST, available since 2010, is the first global web portal for the analysis of IG and TR high throughput sequences. IMGT/HighV-QUEST provides standardized outputs for the characterization of the "IMGT clonotype (AA)" (AA for amino acids) and their comparison in up to one million sequences. Standardized statistical procedures for "IMGT clonotype (AA)" diversity or expression comparisons have recently been described, however, no tool was yet available. IMGT/StatClonotype, a new IMGT(®) tool, evaluates and visualizes statistical significance of pairwise comparisons of IMGT clonotype (AA) diversity or expression, per V (variable), D (diversity), and J (joining) gene of a given IG or TR group, from NGS IMGT/HighV-QUEST statistical output. IMGT/StatClonotype tool is incorporated in the R package "IMGTStatClonotype," with a user-friendly interface. IMGT/StatClonotype is downloadable at IMGT(®) for users to evaluate pairwise comparison of IG and TR NGS statistical output from IMGT/HighV-QUEST and to visualize, on their web browser, the statistical significance of IMGT clonotype (AA) diversity or expression, per gene, the comparative analysis of CDR-IMGT and the V-D-J associations, in immunoprofiles from normal or pathological immune responses.

摘要

为了比较通过下一代测序（NGS）获得的抗原受体免疫球蛋白（IG）和T细胞受体（TR）的复杂免疫组库，对标准化分析和统计程序有着巨大需求。NGS技术可生成数百万个核苷酸序列，并催生了新工具的开发。自2010年起可用的IMGT/HighV-QUEST是首个用于分析IG和TR高通量序列的全球网络门户。IMGT/HighV-QUEST为“IMGT克隆型（氨基酸）”的表征及其在多达一百万个序列中的比较提供标准化输出。最近已描述了用于“IMGT克隆型（氨基酸）”多样性或表达比较的标准化统计程序，然而，尚无可用工具。IMGT/StatClonotype是一种新的IMGT（®）工具，可根据NGS IMGT/HighV-QUEST统计输出，评估并可视化给定IG或TR组中每个V（可变）、D（多样性）和J（连接）基因的IMGT克隆型（氨基酸）多样性或表达的成对比较的统计显著性。IMGT/StatClonotype工具包含在R包“IMGTStatClonotype”中，具有用户友好界面。IMGT/StatClonotype可在IMGT（®）上下载，供用户评估来自IMGT/HighV-QUEST的IG和TR NGS统计输出的成对比较，并在其网络浏览器上可视化每个基因的IMGT克隆型（氨基酸）多样性或表达的统计显著性、CDR-IMGT的比较分析以及V-D-J关联，这些均来自正常或病理性免疫反应的免疫谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/60c0528b64d1/fimmu-07-00339-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/58a0da0e2c4e/fimmu-07-00339-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/1afc2029afb1/fimmu-07-00339-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/168787f7039f/fimmu-07-00339-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/52db51df9262/fimmu-07-00339-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/be3ff26ac1ff/fimmu-07-00339-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/533b4215f62c/fimmu-07-00339-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/60c0528b64d1/fimmu-07-00339-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/58a0da0e2c4e/fimmu-07-00339-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/1afc2029afb1/fimmu-07-00339-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/168787f7039f/fimmu-07-00339-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/52db51df9262/fimmu-07-00339-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/be3ff26ac1ff/fimmu-07-00339-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/533b4215f62c/fimmu-07-00339-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb5c/5016520/60c0528b64d1/fimmu-07-00339-g007.jpg

相似文献

1
IMGT/StatClonotype for Pairwise Evaluation and Visualization of NGS IG and TR IMGT Clonotype (AA) Diversity or Expression from IMGT/HighV-QUEST.IMGT/StatClonotype用于对来自IMGT/HighV-QUEST的NGS免疫球蛋白（IG）和T细胞受体（TR）IMGT克隆型（氨基酸序列）多样性或表达进行成对评估和可视化。
Front Immunol. 2016 Sep 9;7:339. doi: 10.3389/fimmu.2016.00339. eCollection 2016.
2
IMGT/HighV-QUEST Statistical Significance of IMGT Clonotype (AA) Diversity per Gene for Standardized Comparisons of Next Generation Sequencing Immunoprofiles of Immunoglobulins and T Cell Receptors.IMGT/HighV-QUEST：用于免疫球蛋白和T细胞受体下一代测序免疫谱标准化比较的每个基因IMGT克隆型（氨基酸）多样性的统计显著性
PLoS One. 2015 Nov 5;10(11):e0142353. doi: 10.1371/journal.pone.0142353. eCollection 2015.
3
IMGT(®) tools for the nucleotide analysis of immunoglobulin (IG) and T cell receptor (TR) V-(D)-J repertoires, polymorphisms, and IG mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS.用于免疫球蛋白（IG）和T细胞受体（TR）V-（D）-J基因库、多态性及IG突变核苷酸分析的IMGT（®）工具：用于二代测序的IMGT/V-QUEST和IMGT/HighV-QUEST
Methods Mol Biol. 2012;882:569-604. doi: 10.1007/978-1-61779-842-9_32.
4
IG and TR single chain fragment variable (scFv) sequence analysis: a new advanced functionality of IMGT/V-QUEST and IMGT/HighV-QUEST.IG 和 TR 单链片段可变区 (scFv) 序列分析：IMGT/V-QUEST 和 IMGT/HighV-QUEST 的新高级功能。
BMC Immunol. 2017 Jun 26;18(1):35. doi: 10.1186/s12865-017-0218-8.
5
IMGT Immunoinformatics Tools for Standardized V-DOMAIN Analysis.IMGT 免疫信息学工具用于标准化 V-DOMAIN 分析。
Methods Mol Biol. 2022;2453:477-531. doi: 10.1007/978-1-0716-2115-8_24.
6
Correction: IMGT/HighV-QUEST Statistical Significance of IMGT Clonotype (AA) Diversity per Gene for Standardized Comparisons of Next Generation Sequencing Immunoprofiles of Immunoglobulins and T Cell Receptors.更正：IMGT/HighV-QUEST免疫球蛋白和T细胞受体下一代测序免疫谱标准化比较中每个基因的IMGT克隆型（氨基酸）多样性的统计学意义
PLoS One. 2016 Jan 5;11(1):e0146702. doi: 10.1371/journal.pone.0146702. eCollection 2016.
7
ImmunExplorer (IMEX): a software framework for diversity and clonality analyses of immunoglobulins and T cell receptors on the basis of IMGT/HighV-QUEST preprocessed NGS data.免疫探索者（IMEX）：一个基于IMGT/HighV-QUEST预处理的NGS数据对免疫球蛋白和T细胞受体进行多样性和克隆性分析的软件框架。
BMC Bioinformatics. 2015 Aug 12;16:252. doi: 10.1186/s12859-015-0687-9.
8
Coupling of Single Molecule, Long Read Sequencing with IMGT/HighV-QUEST Analysis Expedites Identification of SIV gp140-Specific Antibodies from scFv Phage Display Libraries.单细胞、长读测序与 IMGT/HighV-QUEST 分析的偶联加速了 scFv 噬菌体展示文库中 SIV gp140 特异性抗体的鉴定。
Front Immunol. 2018 Mar 1;9:329. doi: 10.3389/fimmu.2018.00329. eCollection 2018.
9
Comparative analysis of human and mouse immunoglobulin variable heavy regions from IMGT/LIGM-DB with IMGT/HighV-QUEST.利用IMGT/HighV-QUEST对来自IMGT/LIGM-DB的人类和小鼠免疫球蛋白可变重链区域进行比较分析。
Theor Biol Med Model. 2014 Jul 3;11:30. doi: 10.1186/1742-4682-11-30.
10
Pacific Biosciences Sequencing and IMGT/HighV-QUEST Analysis of Full-Length Single Chain Fragment Variable from an Selected Phage-Display Combinatorial Library.来自选定噬菌体展示组合文库的全长单链抗体可变区的太平洋生物科学公司测序及IMGT/HighV-QUEST分析
Front Immunol. 2017 Dec 20;8:1796. doi: 10.3389/fimmu.2017.01796. eCollection 2017.

引用本文的文献

1
Randomized field trial of a therapeutic vaccine against natural infection in dogs and correlates for efficacy.针对犬自然感染的治疗性疫苗的随机现场试验及疗效相关因素
bioRxiv. 2025 May 14:2025.05.12.653529. doi: 10.1101/2025.05.12.653529.
2
Unraveling the Properties of Phage Display Fab Libraries and Their Use in the Selection of Gliadin-Specific Probes by Applying High-Throughput Nanopore Sequencing.解析噬菌体展示 Fab 文库的性质及其在高通量纳米孔测序应用中筛选麦醇溶蛋白特异性探针的作用。
Viruses. 2024 Apr 26;16(5):686. doi: 10.3390/v16050686.
3
Highly Cross-Reactive and Protective Influenza A Virus H3N2 Hemagglutinin- and Neuraminidase-Specific Human Monoclonal Antibodies.

本文引用的文献

1
IMGT/HighV-QUEST Statistical Significance of IMGT Clonotype (AA) Diversity per Gene for Standardized Comparisons of Next Generation Sequencing Immunoprofiles of Immunoglobulins and T Cell Receptors.IMGT/HighV-QUEST：用于免疫球蛋白和T细胞受体下一代测序免疫谱标准化比较的每个基因IMGT克隆型（氨基酸）多样性的统计显著性
PLoS One. 2015 Nov 5;10(11):e0142353. doi: 10.1371/journal.pone.0142353. eCollection 2015.
2
IMGT®, the international ImMunoGeneTics information system® 25 years on.国际免疫基因信息系统（IMGT®）迎来25周年。
Nucleic Acids Res. 2015 Jan;43(Database issue):D413-22. doi: 10.1093/nar/gku1056. Epub 2014 Nov 5.
3
高度交叉反应性和保护性的流感 A 病毒 H3N2 血凝素和神经氨酸酶特异性人源单克隆抗体。
Microbiol Spectr. 2023 Aug 17;11(4):e0472822. doi: 10.1128/spectrum.04728-22. Epub 2023 Jun 15.
4
TCR_Explore: A novel webtool for T cell receptor repertoire analysis.TCR_Explore：一种用于T细胞受体库分析的新型网络工具。
Comput Struct Biotechnol J. 2023 Feb 3;21:1272-1282. doi: 10.1016/j.csbj.2023.01.046. eCollection 2023.
5
Mixed Origins: HIV gp120-Specific Memory Develops from Pre-Existing Memory and Naive B Cells Following Vaccination in Humans.混合起源：HIV gp120 特异性记忆在人类接种疫苗后由预先存在的记忆和幼稚 B 细胞产生。
AIDS Res Hum Retroviruses. 2023 Jul;39(7):350-366. doi: 10.1089/AID.2022.0104. Epub 2023 Mar 22.
6
Antibody Sequence and Structure Analyses Using IMGT: 30 Years of Immunoinformatics.使用 IMGT 进行抗体序列和结构分析：30 年免疫信息学。
Methods Mol Biol. 2023;2552:3-59. doi: 10.1007/978-1-0716-2609-2_1.
7
IMGT Nomenclature of Engineered IGHG Variants Involved in Antibody Effector Properties and Formats.参与抗体效应特性和形式的工程化IGHG变体的IMGT命名法。
Antibodies (Basel). 2022 Oct 18;11(4):65. doi: 10.3390/antib11040065.
8
B-cell receptor physical properties affect relative IgG1 and IgE responses in mouse egg allergy.B细胞受体的物理特性影响小鼠卵过敏中IgG1和IgE的相对反应。
Mucosal Immunol. 2022 Jun;15(6):1375-1388. doi: 10.1038/s41385-022-00567-y. Epub 2022 Sep 16.
9
Purpose-Built Immunoinformatics for BcR IG/TR Repertoire Data Analysis.专为 BcR IG/TR repertoire 数据分析而设计的免疫信息学。
Methods Mol Biol. 2022;2453:585-603. doi: 10.1007/978-1-0716-2115-8_27.
10
IMGT Immunoinformatics Tools for Standardized V-DOMAIN Analysis.IMGT 免疫信息学工具用于标准化 V-DOMAIN 分析。
Methods Mol Biol. 2022;2453:477-531. doi: 10.1007/978-1-0716-2115-8_24.
Differences in the composition of the human antibody repertoire by B cell subsets in the blood.
血液中 B 细胞亚群的人抗体库组成的差异。
Front Immunol. 2014 Mar 19;5:96. doi: 10.3389/fimmu.2014.00096. eCollection 2014.
4
Immunoglobulin and T Cell Receptor Genes: IMGT(®) and the Birth and Rise of Immunoinformatics.免疫球蛋白和 T 细胞受体基因：IMGT(®)和免疫信息学的诞生和兴起。
Front Immunol. 2014 Feb 5;5:22. doi: 10.3389/fimmu.2014.00022. eCollection 2014.
5
IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling.IMGT/HighV QUEST 范式用于 T 细胞受体 IMGT 克隆型多样性和下一代免疫谱分析。
Nat Commun. 2013;4:2333. doi: 10.1038/ncomms3333.
6
Immunoglobulin analysis tool: a novel tool for the analysis of human and mouse heavy and light chain transcripts.免疫球蛋白分析工具：一种用于分析人源和鼠源重链和轻链转录本的新型工具。
Front Immunol. 2012 Jun 28;3:176. doi: 10.3389/fimmu.2012.00176. eCollection 2012.
7
IMGT(®) tools for the nucleotide analysis of immunoglobulin (IG) and T cell receptor (TR) V-(D)-J repertoires, polymorphisms, and IG mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS.用于免疫球蛋白（IG）和T细胞受体（TR）V-（D）-J基因库、多态性及IG突变核苷酸分析的IMGT（®）工具：用于二代测序的IMGT/V-QUEST和IMGT/HighV-QUEST
Methods Mol Biol. 2012;882:569-604. doi: 10.1007/978-1-61779-842-9_32.
8
IMGT-ONTOLOGY 2012.IMGT-ONTOLOGY 2012.
Front Genet. 2012 May 23;3:79. doi: 10.3389/fgene.2012.00079. eCollection 2012.
9
IMGT/GENE-DB: a comprehensive database for human and mouse immunoglobulin and T cell receptor genes.IMGT/GENE-DB：一个关于人类和小鼠免疫球蛋白及T细胞受体基因的综合数据库。
Nucleic Acids Res. 2005 Jan 1;33(Database issue):D256-61. doi: 10.1093/nar/gki010.
10
IMGT standardized criteria for statistical analysis of immunoglobulin V-REGION amino acid properties.IMGT免疫球蛋白V区氨基酸特性统计分析的标准化标准。
J Mol Recognit. 2004 Jan-Feb;17(1):17-32. doi: 10.1002/jmr.647.