Rodrigues Alexandre Dos Santos, Brandão José Henrique Souza Galdino, Bitencourt Jamille de Araújo, Jucá-Chagas Ricardo, Sampaio Iracilda, Schneider Horácio, Affonso Paulo Roberto Antunes de Mello
Department of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Bairro Jequiezinho, 45206-190 Jequié, BA, Brazil.
Institute of Coastal Studies (IECOS), Universidade Federal do Pará, Alameda Leandro Ribeiro s/n, Bairro Aldeia, 68600-000 Bragança, PA, Brazil.
ScientificWorldJournal. 2016;2016:9382613. doi: 10.1155/2016/9382613. Epub 2016 Sep 7.
is a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species, is occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset for , we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species ( spp.) and other fish species () commonly mixed with in street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminating specimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading of and to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.
是一种濒危淡水鱼物种，为巴西东北部一些沿海河流所特有。尽管巴西法律禁止捕捞濒危物种，但在街头市场偶尔仍能发现，深受当地居民喜爱。为了为提供可靠的DNA条形码数据集，我们比较了新鲜、冷冻和盐渍标本中细胞色素c氧化酶亚基I（COI）的线粒体序列。系统发育相关物种（属）以及在街头市场常与混在一起的其他鱼类物种（）也被纳入，以测试分子鉴定的效率。尽管一些商业样本的保存过程存在差异且有严重变质情况，但仍获得了高质量的COI序列，且能有效区分标本。此外，虽然孔塔斯和阿尔马达河流域的种群似乎构成一个单一的进化谱系，但卡舒埃拉河的标本在基因上存在差异，表明存在种群结构。因此，DNA条形码已被证明有助于追踪的非法交易并管理濒危种群，这应侧重于保护不同的遗传种群并减轻其分布范围内的人类影响。
