Gill Samantha K, Price Mike, Costa Ricardo J S
Education and Professional Development, British Dietetic Association, Birmingham, UK.
Applied Biology and Exercise Sciences Research Centre, School of Life Sciences, Coventry University, Coventry, UK.
Eur J Oral Sci. 2016 Oct;124(5):447-453. doi: 10.1111/eos.12294. Epub 2016 Sep 27.
The aim of the current study was to determine if unstimulated saliva flow (measured in μl min ) is affected by different durations of sample collection and by temperatures of mouthrinse water used before sample collection. In randomized order, participants provided 10 samples of unstimulated saliva at time points ranging from 1 to 6 min after rinsing with different temperatures of water (10, 20, and 30°C). Data were analysed by one-way anova with post-hoc tests. Test-retest reliability was assessed using Bland-Altman plots and correlation coefficients. A larger volume of saliva was obtained over a longer collection time. No significant difference in saliva flow rate was observed between collection times [mean: 364 (95% CI: 332-397) μl min ]. Although rinsing with different temperatures of mouthrinse water resulted in no significant difference in saliva flow rates as a result of the mouthrinse water temperatures, 60% of the participants had a higher saliva flow rate after rinsing with mouthrinse water at a temperature of 10°C compared with mouthrinse water at 20 and 30°C, suggesting large individual variation (range: 24-420 μl min ). These findings provide justification for using saliva collection times of 1-6 min during sampling of unstimulated saliva. The large individual variations in saliva flow rate in response to different mouthrinse water temperatures suggest that standardization, control, and reporting of mouthrinse water temperature is warranted.
本研究的目的是确定未刺激唾液流量（以微升/分钟为单位测量）是否受不同样本采集时长以及样本采集前使用的漱口水温度的影响。参与者按照随机顺序，在使用不同温度（10、20和30°C）的水漱口后1至6分钟的时间点提供10份未刺激唾液样本。数据通过单因素方差分析及事后检验进行分析。使用布兰德-奥特曼图和相关系数评估重测信度。在更长的采集时间内获得了更大体积的唾液。在采集时间之间未观察到唾液流速有显著差异[均值：364（95%CI：332 - 397）微升/分钟]。尽管由于漱口水温度不同，用不同温度的漱口水漱口后唾液流速没有显著差异，但与用20°C和30°C漱口水漱口相比，60%的参与者在用10°C漱口水漱口后唾液流速更高，这表明个体差异很大（范围：24 - 420微升/分钟）。这些发现为在采集未刺激唾液样本时使用1至6分钟的唾液采集时间提供了依据。唾液流速对不同温度漱口水的个体差异很大，这表明漱口水温度的标准化、控制和报告是有必要的。
