Chen Yung-Ju, Myracle Angela D, Wallig Matthew A, Jeffery Elizabeth H
Department of Food Science and Human Nutrition, University of Illinois, 905 South Goodwin Avenue, Urbana, IL 61801, USA.
Department of Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61802, USA.
J Funct Foods. 2016 Jun;24:57-62. doi: 10.1016/j.jff.2016.03.028. Epub 2016 Apr 12.
Western-style high fat, high sugar diets are associated with non-alcoholic fatty liver disease (NAFLD) and increased liver cancer risk. Sulforaphane from broccoli may protect against these. Previously we initiated broccoli feeding to mice prior to exposure to the hepatocarcinogen diethylnitrosamine (DEN), and saw protection against NAFLD and liver cancer. Here we administered DEN to unweaned mice, initiating broccoli feeding two weeks later, to determine if broccoli protects against cancer progression. Specifically, male 15-day-old C57BL/6J mice were given DEN and placed on a Western or Western+10%Broccoli diet from the age of 4 weeks through 7 months. Dietary broccoli decreased hepatic triacylglycerols, NAFLD, liver damage and tumour necrosis factor by month 5 without changing body weight or relative liver weight, but did not slow carcinogenesis, seen in 100% of mice. We conclude that broccoli, a good source of sulforaphane, slows progression of hepatic lipidosis, but not tumourigenesis in this robust model.
西式高脂肪、高糖饮食与非酒精性脂肪性肝病（NAFLD）以及肝癌风险增加有关。西兰花中的萝卜硫素可能对这些情况起到预防作用。此前我们在给小鼠暴露于肝癌致癌物二乙基亚硝胺（DEN）之前就开始用西兰花喂养它们，发现对NAFLD和肝癌有预防作用。在此我们给未断奶的小鼠施用DEN，两周后开始用西兰花喂养，以确定西兰花是否能预防癌症进展。具体而言，15日龄的雄性C57BL/6J小鼠接受DEN处理，并从4周龄到7个月龄置于西式或西式+10%西兰花饮食中。到第5个月时，饮食中的西兰花降低了肝脏三酰甘油、NAFLD、肝脏损伤和肿瘤坏死因子，而体重或相对肝脏重量没有变化，但并没有减缓致癌作用，100%的小鼠都出现了致癌情况。我们得出结论，在这个强有力的模型中，西兰花作为萝卜硫素的良好来源，减缓了肝脏脂肪变性的进展，但没有减缓肿瘤发生。
