Goudarzi Mehdi, Fazeli Maryam, Goudarzi Hossein, Azad Mehdi, Seyedjavadi Sima Sadat
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
WHO Collaborating Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, IR Iran.
Jundishapur J Microbiol. 2016 Jul 2;9(7):e35685. doi: 10.5812/jjm.35685. eCollection 2016 Jul.
The incidence of nosocomial Staphylococcus aureus infection is increasing annually and becoming a true global challenge. The pattern of Staphylococcus aureus protein A (spa) types in different geographic regions is diverse.
This study determined the prevalence of methicillin-resistant S. aureus and different spa types in S. aureus clinical isolates.
During a six-month period, 90 S. aureus isolates were recovered from 320 clinical specimens. The in vitro susceptibility of various S. aureus isolates to 16 antibiotic discs was assessed using the Kirby-Bauer disk diffusion method. Molecular typing was carried out with S. aureus protein A typing via polymerase chain reaction.
The frequency of methicillin-resistant S. aureus in our study was 88.9%. Twenty-three (25.5%) isolates were positive for panton-valentine leukocidin encoding genes. S. aureus presented a high resistance rate to ampicillin (100%) and penicillin (100%). No resistance was observed to vancomycin, teicoplanin, or linezolid. The rates of resistance to the majority of antibiotics tested varied between 23.3% and 82.2%. The rate of multidrug resistance among these clinical isolates was 93.3%. The 90 S. aureus isolates were classified into five S. aureus protein A types: t037 (33.3%), t030 (22.2%), t790 (16.7%), t969 (11.1%), and t044 (7.7%). Eight (8.9%) isolates were not typable using the S. aureus protein A typing method.
We report a high methicillin-resistant S. aureus rate in our hospital. Additionally, t030 and t037 were the predominant spa-types among hospital-associated S. aureus. Our findings emphasize the need for continuous surveillance to prevent the dissemination of multidrug resistance among different S. aureus protein A types in Iran.
医院内金黄色葡萄球菌感染的发生率逐年上升，成为一个真正的全球性挑战。不同地理区域的金黄色葡萄球菌蛋白A（spa）类型模式各不相同。
本研究确定了金黄色葡萄球菌临床分离株中耐甲氧西林金黄色葡萄球菌和不同spa类型的流行情况。
在六个月的时间里，从320份临床标本中分离出90株金黄色葡萄球菌。采用 Kirby-Bauer 纸片扩散法评估各种金黄色葡萄球菌分离株对16种抗生素纸片的体外敏感性。通过聚合酶链反应对金黄色葡萄球菌进行蛋白A分型，进行分子分型。
在我们的研究中，耐甲氧西林金黄色葡萄球菌的频率为88.9%。23株（25.5%）分离株的杀白细胞素编码基因呈阳性。金黄色葡萄球菌对氨苄西林（100%）和青霉素（100%）呈现出高耐药率。未观察到对万古霉素、替考拉宁或利奈唑胺的耐药情况。对大多数测试抗生素的耐药率在23.3%至82.2%之间。这些临床分离株中的多重耐药率为93.3%。90株金黄色葡萄球菌分离株被分为五种金黄色葡萄球菌蛋白A类型：t037（33.3%）、t030（22.2%）、t790（16.7%）、t969（11.1%）和t044（7.7%）。8株（8.9%）分离株使用金黄色葡萄球菌蛋白A分型方法无法分型。
我们报告了我院耐甲氧西林金黄色葡萄球菌的高发生率。此外，t030和t037是医院相关金黄色葡萄球菌中主要的spa类型。我们的研究结果强调了持续监测以防止伊朗不同金黄色葡萄球菌蛋白A类型之间多重耐药性传播的必要性。
