Bojar Iwona, Pinkas Jarosław, Wierzbińska-Stępniak Anna, Raczkiewicz Dorota, Owoc Alfred, Gujski Mariusz
Department of Women's Health, Institute of Rural Health in Lublin, Lublin, Poland.
School of Public Health, Center for Postgraduate Medical Education, Warsaw, Poland.
Med Sci Monit. 2016 Sep 28;22:3469-3478. doi: 10.12659/msm.901247.
BACKGROUND The goal of this study was to investigate the relationship between cognitive functions and the level of endogenous estradiol in postmenopausal women, according to which estrogen receptor α (ERα) polymorphism the woman carries. MATERIAL AND METHODS The study group consisted of 210 women. The inclusion criteria were: minimum 2 years after the last menstruation, FSH concentration 30 U/ml, and no dementia signs on Montreal Cognitive Assessment (MoCA). A computerized battery of Central Nervous System Vital Signs (CNS VS) test was used to diagnose cognitive functions. Genotyping of the ERa polymorphism was performed using a polymerase chain reaction and restriction enzymes (PCR-RFLP). Blood plasma was tested for FSH and estradiol (E2). Statistical analysis was performed using STATISTICA software. RESULTS A relationship was confirmed between standard scores for 3 cognitive functions: general memory, verbal memory, and processing speed, and the XbaI polymorphism in the women in the study. In the group of women with genotype TT PvuII, significant positive relationships were observed between the concentration of E2 and the standard scores of 3 cognitive functions: general memory, verbal memory, and processing speed. In the group of women with genotype TC PvuII, significant negative correlations were found between the concentration of E2 and the standard scores of 4 cognitive functions: NCI, general memory, verbal memory, and processing speed. CONCLUSIONS ERα polymorphism exerted an effect on the interaction between the concentration of estradiol and the results for cognitive functions. The concentration of estradiol did not depend on Xba1 and PvuII polymorphisms. The results for cognitive functions depended on which Xba1 polymorphism the woman carried.
背景 本研究的目的是根据绝经后女性所携带的雌激素受体α（ERα）多态性，调查认知功能与内源性雌二醇水平之间的关系。
材料与方法 研究组由210名女性组成。纳入标准为：末次月经后至少2年，促卵泡激素（FSH）浓度≥30 U/ml，且蒙特利尔认知评估（MoCA）无痴呆迹象。使用一套计算机化的中枢神经系统生命体征（CNS VS）测试来诊断认知功能。采用聚合酶链反应和限制性内切酶（PCR-RFLP）对ERα多态性进行基因分型。检测血浆中的FSH和雌二醇（E2）。使用STATISTICA软件进行统计分析。
结果 研究证实，3项认知功能的标准分数（一般记忆、言语记忆和处理速度）与研究中女性的XbaI多态性之间存在关联。在基因型为TT PvuII的女性组中，观察到E2浓度与3项认知功能的标准分数（一般记忆、言语记忆和处理速度）之间存在显著正相关。在基因型为TC PvuII的女性组中，发现E2浓度与4项认知功能的标准分数（NCI、一般记忆、言语记忆和处理速度）之间存在显著负相关。
结论 ERα多态性对雌二醇浓度与认知功能结果之间的相互作用产生影响。雌二醇浓度不依赖于Xba1和PvuII多态性。认知功能结果取决于女性所携带的Xba1多态性。
