Ikado Yuichiro, Obinata Daisuke, Matsumoto Taro, Murata Yasutaka, Kano Koichiro, Fukuda Noboru, Yamaguchi Kenya, Takahashi Satoru
Department of Urology, Nihon University School of Medicine, Tokyo, Japan.
Division of Cell Regeneration and Transplantation, Department of Functional Morphology, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Int Urol Nephrol. 2016 Dec;48(12):1951-1960. doi: 10.1007/s11255-016-1426-5. Epub 2016 Sep 28.
Autologous cells potentially provide an ideal injectable substance for management in vesicoureteral reflux (VUR). The aim of this study is to examine the effects of mature adipocyte-derived dedifferentiated fat (DFAT) cell transplantation on VUR in a rat bladder pressurization-induced VUR model.
To create VUR, Sprague-Dawley rats underwent urethral clamping and placement of cystostomy followed by intravesical pressurization. Rat DFAT cells (1 × 10 cells, DFAT group, n = 5) or saline (control group, n = 5) was then injected into the bilateral vesicoureteral junctions. Two weeks later, VUR grade was evaluated on cystography. The number of apoptotic cells in the renal pelvic urothelium, the ureteral inner/outer diameter ratio and the area of connective tissue in the posterior bladder wall were measured.
The reflux grade in the DFAT group was significantly lower than that in the control group. The number of apoptotic cells in the renal pelvic urothelium, ureteral inner/outer diameter ratio and connective tissue area in DFAT group were significantly lower in comparison with the control group.
DFAT cell transplantation improved VUR and exerted nephroprotective effects in a rat VUR model.
自体细胞可能为膀胱输尿管反流（VUR）的治疗提供一种理想的可注射物质。本研究的目的是在大鼠膀胱加压诱导的VUR模型中，研究成熟脂肪细胞来源的去分化脂肪（DFAT）细胞移植对VUR的影响。
为制造VUR，对Sprague-Dawley大鼠进行尿道夹闭和膀胱造口术，随后进行膀胱内加压。然后将大鼠DFAT细胞（1×10个细胞，DFAT组，n = 5）或生理盐水（对照组，n = 5）注入双侧膀胱输尿管连接处。两周后，通过膀胱造影评估VUR分级。测量肾盂尿路上皮中的凋亡细胞数量、输尿管内外径比值以及膀胱后壁结缔组织面积。
DFAT组的反流分级显著低于对照组。与对照组相比，DFAT组肾盂尿路上皮中的凋亡细胞数量、输尿管内外径比值和结缔组织面积显著降低。
DFAT细胞移植改善了大鼠VUR模型中的VUR，并发挥了肾保护作用。
