Ebling W F, Mills-Williams L, Harapat S R, Stanski D R
Department of Anesthesia, Stanford University School of Medicine, CA 94305.
J Chromatogr. 1989 May 30;490(2):339-53. doi: 10.1016/s0378-4347(00)82792-9.
A sensitive, selective and reproducible high-performance liquid chromatographic assay of thiopental concentrations in twelve rat tissues was developed using thiamylal as the internal standard. Samples were homogenized in phosphate buffer, extracted into pentane and chromatographed on a microparticulate octadecyl reversed-phase column using ultraviolet detection at 290 nm. A simple digestive step with collagenase prior to homogenization facilitated analysis of thiobarbiturate in skin. Thiopental extraction recovery from fat exceeded 90%. Assay sensitivity was greater than 1 microgram/ml for tissue and plasma samples as small as 50 microliters. This assay has been applied to physiologic pharmacokinetic studies. The paper also presents typical concentration-time profiles of thiopental in four tissues taken from 74 rats given 20 mg/kg thiopental.
采用硫喷妥钠作为内标，建立了一种灵敏、特异且可重复的高效液相色谱法，用于测定12种大鼠组织中的硫喷妥浓度。样品在磷酸盐缓冲液中匀浆，用戊烷萃取，然后在微粒十八烷基反相柱上进行色谱分析，采用290nm紫外检测。在匀浆前用胶原酶进行简单的消化步骤有助于皮肤中硫代巴比妥酸盐的分析。脂肪中硫喷妥的萃取回收率超过90%。对于小至50微升的组织和血浆样品，该测定法的灵敏度大于1微克/毫升。该测定法已应用于生理药代动力学研究。本文还给出了74只给予20mg/kg硫喷妥的大鼠的四种组织中硫喷妥的典型浓度-时间曲线。
