Yashiki M, Kojima T, Okamoto I
Forensic Sci Int. 1987 Mar;33(3):169-75. doi: 10.1016/0379-0738(87)90125-3.
A simple, rapid and accurate method to extract and clean thiopental in biological materials rich in fat was developed by using a separation column packed with Extrelut and Florisil. A Quantitative determination of thiopental by means of a gas chromatograph equipped with a flame photometric sulfur detector was attempted. The calibration curve was linear over the range of 5-200 micrograms/ml. Extraction of replicate tissues involving 3 micrograms of thiopental resulted in a recovery of the 99.7-101.8% range and a coefficient of deviation of the 0.3-1.9% range. Replicate extraction of five tissues from rats treated with thiopental resulted in a coefficient of deviation of below 6%. In rats given 40 mg/kg of thiopental and sacrificed after 5 min, the thiopental levels in fat were found to be higher after a slow intravenous injection than after a quick injection. The same tendency, however, was not observed in other tissues. It seems that the rate of intravenous thiopental injections might be estimated by comparison with thiopental levels in fat tissues.
通过使用填充有硅藻土和弗罗里硅土的分离柱，开发了一种简单、快速且准确的方法来提取和净化富含脂肪的生物材料中的硫喷妥钠。尝试使用配备火焰光度硫检测器的气相色谱仪对硫喷妥钠进行定量测定。校准曲线在5-200微克/毫升范围内呈线性。对含有3微克硫喷妥钠的重复组织进行提取，回收率在99.7%-101.8%范围内，偏差系数在0.3%-1.9%范围内。对用硫喷妥钠处理的大鼠的五个组织进行重复提取，偏差系数低于6%。给大鼠注射40毫克/千克硫喷妥钠并在5分钟后处死，发现缓慢静脉注射后脂肪中的硫喷妥钠水平高于快速注射后。然而，在其他组织中未观察到相同趋势。似乎可以通过与脂肪组织中的硫喷妥钠水平进行比较来估计硫喷妥钠静脉注射的速率。
