Hurley S S, Splitter G A, Welch R A
Department of Natural Resources, Madison, Wisconsin 53707.
J Clin Microbiol. 1989 Jul;27(7):1582-7. doi: 10.1128/jcm.27.7.1582-1587.1989.
A DNA probe, M13 mpHAW71, that detects Mycobacterium paratuberculosis in the fecal material of infected animals was developed for use in the diagnosis of Johne's disease. The probe detected as few as 10(5) M. paratuberculosis when hybridized under stringent conditions to total genomic DNA purified from bovine fecal material. When the probe was used diagnostically, it did not differentiate members of the Mycobacterium avium-M. intracellulare-M. paratuberculosis complex. Compared with culturing, the DNA probe identified 34.4% more mycobacterium-containing fecal samples, and testing took only 72 h to complete.
一种用于诊断副结核的DNA探针M13 mpHAW71被开发出来，它可检测感染动物粪便中的副结核分枝杆菌。在严格条件下，该探针与从牛粪中纯化的全基因组DNA杂交时，可检测到低至10(5)个副结核分枝杆菌。当该探针用于诊断时，它无法区分鸟分枝杆菌-胞内分枝杆菌-副结核分枝杆菌复合体的成员。与培养法相比，DNA探针能多识别出34.4%的含分枝杆菌粪便样本，且检测只需72小时就能完成。
