副结核分枝杆菌特异性重复DNA序列的鉴定
Identification of a repetitive DNA sequence specific to Mycobacterium paratuberculosis.
作者信息
Collins D M, Gabric D M, De Lisle G W
机构信息
Central Animal Health Laboratory, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
出版信息
FEMS Microbiol Lett. 1989 Jul 15;51(1):175-8. doi: 10.1016/0378-1097(89)90503-x.
A 0.2-kb DNA sequence specific to Mycobacterium paratuberculosis, the causative organism of Johne's disease, was isolated from a partial genomic library. The sequence was part of a larger repetitive DNA element and was present in strains of M. paratuberculosis from cattle, sheep, goat, deer and also a woman with Crohn's disease but not in M. paratuberculosis strain 18. The sequence was not present in strains of 19 other mycobacterial species including 31 reference serotype strains of the M. avium-M. intracellular-M. scrofulaceum (MAIS) complex, some strains of which are closely related to M. paratuberculosis. The sequence may be useful for developing a diagnostic test for Johne's disease.
从一个部分基因组文库中分离出了一段0.2千碱基对的、对副结核分枝杆菌（约翰氏病的致病微生物）具有特异性的DNA序列。该序列是一个更大的重复DNA元件的一部分，存在于来自牛、羊、山羊、鹿的副结核分枝杆菌菌株中，也存在于一名患有克罗恩病的女性患者体内的副结核分枝杆菌菌株中，但不存在于副结核分枝杆菌18菌株中。该序列不存在于其他19种分枝杆菌菌株中，包括鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌（MAIS）复合体的31个参考血清型菌株，其中一些菌株与副结核分枝杆菌密切相关。该序列可能有助于开发一种针对约翰氏病的诊断测试。
Zotero 插件