Vandamme P, Falsen E, Pot B, Hoste B, Kersters K, De Ley J
Laboratorium voor Microbiologie en Microbiële Genetica, Rijksuniversiteit Gent, Belgium.
J Clin Microbiol. 1989 Aug;27(8):1775-81. doi: 10.1128/jcm.27.8.1775-1781.1989.
EF (E. Falsen) group 22, a group of Campylobacter strains sorted out by routine immunotyping among unidentified or misidentified human nonoral clinical specimens, was characterized by numerical analysis of gel electrophoretic protein profiles and immunotyping. The protein electrophoretic and immunotyping analyses, DNA:DNA hybridizations, and the DNA base composition demonstrated unambiguously that all EF group 22 strains belong to Campylobacter concisus. EF group 22 strains have DNA binding values of at least 42% with the type strain of C. concisus, showing a considerable degree of genomic heterogeneity. The isolation from blood, esophagus, stomach, duodenum, and feces of humans in association with different gastrointestinal disorders considerably extends the clinical significance of this species. Our results indicate that sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunotyping are excellent tools for the identification of the fastidious C. concisus strains and relatives.
EF（E. Falsen）第22组是一组弯曲杆菌菌株，通过常规免疫分型从未鉴定或错误鉴定的人类非口腔临床标本中筛选出来，通过凝胶电泳蛋白质谱的数值分析和免疫分型进行特征描述。蛋白质电泳和免疫分型分析、DNA:DNA杂交以及DNA碱基组成明确表明，所有EF第22组菌株均属于简明弯曲杆菌。EF第22组菌株与简明弯曲杆菌模式菌株的DNA结合值至少为42%，显示出相当程度的基因组异质性。从患有不同胃肠道疾病的人类血液、食管、胃、十二指肠和粪便中分离出该菌，大大扩展了该菌种的临床意义。我们的结果表明，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫分型是鉴定苛求的简明弯曲杆菌菌株及其相关菌的优秀工具。
