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通过聚丙烯酰胺平板凝胶中的蛋白质条带模式对弯曲杆菌属菌种进行鉴别。

Differentiation of Campylobacter species by protein banding patterns in polyacrylamide slab gels.

作者信息

Ferguson D A, Lambe D W

出版信息

J Clin Microbiol. 1984 Sep;20(3):453-60. doi: 10.1128/jcm.20.3.453-460.1984.

DOI:10.1128/jcm.20.3.453-460.1984
PMID:6490829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271349/
Abstract

Soluble protein extracts of 37 catalase-positive strains of Campylobacter species were examined by polyacrylamide slab gel electrophoresis (PAGE). Electrophoretic banding patterns showed good correlation with biochemical tests and with available DNA homology data in distinguishing species of Campylobacter but did not differentiate subspecies or biotypes. PAGE patterns indicated that Campylobacter coli is a distinct species. Furthermore, the PAGE patterns indicated that C. jejuni and nalidixic acid-resistant thermophilic Campylobacter species (C. laridis) are each distinct species. The protein banding patterns of C. fetus subsp. venerealis and C. fetus subsp. fetus strains were distinctly different from those of the three thermophilic species.

摘要

采用聚丙烯酰胺平板凝胶电泳(PAGE)对37株过氧化氢酶阳性弯曲杆菌属菌株的可溶性蛋白提取物进行了检测。电泳条带模式在区分弯曲杆菌属物种方面与生化试验及现有的DNA同源性数据显示出良好的相关性,但无法区分亚种或生物型。PAGE模式表明空肠弯曲菌是一个独特的物种。此外,PAGE模式表明空肠弯曲菌和耐萘啶酸嗜热弯曲菌物种(拉氏弯曲菌)均为独特的物种。胎儿弯曲菌性病亚种和胎儿弯曲菌胎儿亚种菌株的蛋白条带模式与三种嗜热物种的明显不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/db4da5878cdb/jcm00122-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/4a6a063c793a/jcm00122-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/5aedae4cd056/jcm00122-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/f8e9d755d3d2/jcm00122-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/1b5e800cb1e5/jcm00122-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/7151fd8e4c89/jcm00122-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/db4da5878cdb/jcm00122-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/4a6a063c793a/jcm00122-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/5aedae4cd056/jcm00122-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/f8e9d755d3d2/jcm00122-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/1b5e800cb1e5/jcm00122-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/7151fd8e4c89/jcm00122-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bac2/271349/db4da5878cdb/jcm00122-0179-a.jpg

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本文引用的文献

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Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras.用于研究细菌菌群的可溶性蛋白质的聚丙烯酰胺板凝胶电泳。
Appl Environ Microbiol. 1980 Apr;39(4):900-7. doi: 10.1128/aem.39.4.900-907.1980.
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Grouping of lactic streptococci by gel electrophoresis of soluble cell extracts.采用可溶性细胞提取物凝胶电泳对乳链球菌进行分组。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Evaluation of API Campy in comparison with conventional methods for identification of thermophilic campylobacters.将API弯曲菌检测系统与传统方法用于嗜热弯曲菌鉴定的比较评估。
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Isolation of Helicobacter pylori from saliva.从唾液中分离幽门螺杆菌。
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Atypical campylobacters associated with gastroenteritis.与肠胃炎相关的非典型弯曲杆菌。
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Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus.从肠胃炎病例中鉴定出22组弯曲杆菌属为空肠弯曲菌。
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Human infections with Vibrio fetus and a closely related vibrio.人类感染胎儿弧菌及一种密切相关的弧菌。
J Infect Dis. 1957 Sep-Oct;101(2):119-28. doi: 10.1093/infdis/101.2.119.
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Campylobacter fetus ssp jejuni: isolation from patients with gastroenteritis.胎儿弯曲菌空肠亚种:从肠胃炎患者中分离得到
South Med J. 1981 Feb;74(2):157-61. doi: 10.1097/00007611-198102000-00008.
6
Serology of Campylobacter fetus ss. jejuni )"related" campylobacters). Demonstration of strain-specific and interstrain-related antigens by immunoelectrophoresis and co-agglutination.胎儿弯曲菌空肠亚种(“相关”弯曲菌)的血清学。通过免疫电泳和协同凝集法显示菌株特异性和菌株间相关抗原。
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7
Cellular fatty acid and soluble protein composition of Actinobacillus actinomycetemcomitans and related organisms.伴放线放线杆菌及相关微生物的细胞脂肪酸和可溶性蛋白质组成
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Deoxyribonucleic acid sequence relatedness between thermophilic members of the genus Campylobacter.弯曲杆菌属嗜热菌成员之间的脱氧核糖核酸序列相关性。
J Gen Microbiol. 1982 Nov;128(11):2515-22. doi: 10.1099/00221287-128-11-2515.
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Differentiation of enteropathogenic Campylobacter.肠道致病性弯曲杆菌的鉴别
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Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence.通过直接免疫荧光法确定的空肠弯曲菌、结肠弯曲菌和胎儿弯曲菌的血清群。
J Clin Microbiol. 1983 Mar;17(3):529-38. doi: 10.1128/jcm.17.3.529-538.1983.