Ferguson D A, Lambe D W
J Clin Microbiol. 1984 Sep;20(3):453-60. doi: 10.1128/jcm.20.3.453-460.1984.
Soluble protein extracts of 37 catalase-positive strains of Campylobacter species were examined by polyacrylamide slab gel electrophoresis (PAGE). Electrophoretic banding patterns showed good correlation with biochemical tests and with available DNA homology data in distinguishing species of Campylobacter but did not differentiate subspecies or biotypes. PAGE patterns indicated that Campylobacter coli is a distinct species. Furthermore, the PAGE patterns indicated that C. jejuni and nalidixic acid-resistant thermophilic Campylobacter species (C. laridis) are each distinct species. The protein banding patterns of C. fetus subsp. venerealis and C. fetus subsp. fetus strains were distinctly different from those of the three thermophilic species.
采用聚丙烯酰胺平板凝胶电泳(PAGE)对37株过氧化氢酶阳性弯曲杆菌属菌株的可溶性蛋白提取物进行了检测。电泳条带模式在区分弯曲杆菌属物种方面与生化试验及现有的DNA同源性数据显示出良好的相关性,但无法区分亚种或生物型。PAGE模式表明空肠弯曲菌是一个独特的物种。此外,PAGE模式表明空肠弯曲菌和耐萘啶酸嗜热弯曲菌物种(拉氏弯曲菌)均为独特的物种。胎儿弯曲菌性病亚种和胎儿弯曲菌胎儿亚种菌株的蛋白条带模式与三种嗜热物种的明显不同。