Costa Juan Gabriel, Duré Andrea Belén
Laboratorio de Microbiología, Hospital provincial del Centenario, Rosario C.P.:2000, Santa Fe, Argentina; Cátedra de Fisicoquímica de la Facultad de Bioquímica y Ciencias Biológicas de Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe C.P.: 3000, Santa Fe, Argentina.
Laboratorio de Biología Celular y Molecular, Instituto de Ciencias Veterinarias del Litoral, CONICET, Facultad de Ciencias Veterinarias de la Universidad Nacional del Litoral, R.P. Kreder 2805, Esperanza C.P.: 3080, Santa Fe, Argentina.
Microb Pathog. 2016 Nov;100:229-236. doi: 10.1016/j.micpath.2016.09.021. Epub 2016 Sep 28.
In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results.
在本研究中，通过间接ELISA和亲和力测定对两个弓形虫GRA8蛋白序列进行了检测，以区分急性和慢性弓形虫感染。使用名为GRA8B的抗原，检测IgG浓度时灵敏度达到79.7%，特异性达到84.1%；检测IgA浓度时灵敏度为71.2%，特异性为68.3%。本研究首次报道了通过ELISA检测GRA8的IgA以区分感染阶段。遗憾的是，间接ELISA检测IgM在区分感染阶段方面效果不佳。此外，本研究首次报道GRA8蛋白可通过测量IgG抗体亲和力来辅助区分急性期和慢性期感染，在该技术中我们分别获得了85.71%和100%的灵敏度和特异性。最后，使用计算机工具来解释我们免疫化学结果中的差异。
