Costa Juan Gabriel, Vilariño María Julia
Cátedra de Fisicoquímica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, Santa Fe, Argentina.
Hospital Escuela Eva Perón, Av. San Martín 1645, Granadero Baigorria, Santa Fe, Argentina.
J Microbiol Methods. 2018 Jun;149:9-13. doi: 10.1016/j.mimet.2018.04.015. Epub 2018 Apr 21.
In this work we present a novel methodology to differentiate the phases of toxoplasmosis infection: the "semiquantitative Dot Blot". It is a simple technique that does not require expensive equipment, does not involve a long technique development, and can be used in a low-complexity laboratory. In this study, two recombinant sequences of Toxoplasma gondii GRA8 antigen were used, and specific IgG antibodies were detected in selected patient samples. This method makes it possible to obtain a score for each serum and define whether the patient is in the acute or chronic phase of the infection. The sensitivity and specificity results varied depending on the antigenic sequence used. With GRA8A, 62.1% and 72.7% were obtained, while with GRA8B, 82.8% and 72.1% were obtained, respectively. Although the sensitivity and specificity values were not close to 100%, they were similar to those reported with the same antigens in ELISA. Therefore, this quantitative technique would be a good alternative to ELISA.
在这项工作中，我们提出了一种区分弓形虫感染阶段的新方法：“半定量斑点印迹法”。这是一种简单的技术，不需要昂贵的设备，不涉及漫长的技术开发过程，并且可以在低复杂度的实验室中使用。在本研究中，使用了两种弓形虫GRA8抗原的重组序列，并在选定的患者样本中检测到了特异性IgG抗体。该方法能够为每份血清获得一个分数，并确定患者处于感染的急性期还是慢性期。敏感性和特异性结果因所使用的抗原序列而异。使用GRA8A时，敏感性和特异性分别为62.1%和72.7%，而使用GRA8B时，分别为82.8%和72.1%。尽管敏感性和特异性值未接近100%，但与ELISA中使用相同抗原所报告的值相似。因此，这种定量技术将是ELISA的一个很好的替代方法。
