Song Min, He Gang, Wang Yan, Pang Xueli, Zhang Bo
Department of Medical Genetics, Third Military Medical University; Department of neurology, the second Affiliated Hospital of Chongqing Medical University.
Department of Medical Genetics, Third Military Medical University.
J Cancer. 2016 Jul 26;7(12):1694-1700. doi: 10.7150/jca.15086. eCollection 2016.
Histone deacetylationase 1 () is ubiquitously expressed in various cell lines and tissues and play an important role of regulation gene expression. Overexpression of has been observed in various types of cancers, which indicated that it might be a target for cancer therapy. To test inhibition for cancer treatment, the gene expression of was knockdown mediated by a lentivirus system. Our data showed the gene expression of could be efficiently knockdown by RNAi mediated by lentivirus in esophageal carcinoma EC109 cells. Knockdown of led to significant decrease of cell growth and altered cell cycle distribution. The result of transwell assay showed that the numbers of cells travelled through the micropore membrane was significantly decreased as expression was knockdown. Moreover, knockdown inhibited the migration of EC109 cells as determining by scratch test. Additionally, enhancement of cisplatin-stimulated apoptosis was detected by knockdown. Our data suggested inhibition of expression by lentivirus mediated shRNA might be further applied for esophageal cancer chemotherapy.
组蛋白去乙酰化酶1（）在各种细胞系和组织中广泛表达，并在调节基因表达中发挥重要作用。在各种类型的癌症中均观察到的过表达，这表明它可能是癌症治疗的一个靶点。为了测试对癌症治疗的抑制作用，通过慢病毒系统介导敲低的基因表达。我们的数据表明，慢病毒介导的RNAi可在食管癌EC109细胞中有效敲低的基因表达。敲低导致细胞生长显著降低，并改变细胞周期分布。Transwell实验结果表明，随着表达的敲低，穿过微孔膜的细胞数量显著减少。此外，划痕试验表明敲低抑制了EC109细胞的迁移。另外，通过敲低检测到顺铂刺激的细胞凋亡增强。我们的数据表明，慢病毒介导的shRNA抑制表达可能进一步应用于食管癌化疗。
