Lentivirus-mediated Knockdown of HDAC1 Uncovers Its Role in Esophageal Cancer Metastasis and Chemosensitivity.

Histone deacetylationase 1 () is ubiquitously expressed in various cell lines and tissues and play an important role of regulation gene expression. Overexpression of has been observed in various types of cancers, which indicated that it might be a target for cancer therapy. To test inhibition for cancer treatment, the gene expression of was knockdown mediated by a lentivirus system. Our data showed the gene expression of could be efficiently knockdown by RNAi mediated by lentivirus in esophageal carcinoma EC109 cells. Knockdown of led to significant decrease of cell growth and altered cell cycle distribution. The result of transwell assay showed that the numbers of cells travelled through the micropore membrane was significantly decreased as expression was knockdown. Moreover, knockdown inhibited the migration of EC109 cells as determining by scratch test. Additionally, enhancement of cisplatin-stimulated apoptosis was detected by knockdown. Our data suggested inhibition of expression by lentivirus mediated shRNA might be further applied for esophageal cancer chemotherapy.